Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1084176..1084903 Replicon chromosome
Accession NZ_CP082357
Organism Escherichia coli strain VCSW7

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag K7X31_RS05135 Protein ID WP_000547555.1
Coordinates 1084176..1084487 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1M2G9D6
Locus tag K7X31_RS05140 Protein ID WP_001600394.1
Coordinates 1084484..1084903 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7X31_RS05110 (1080111) 1080111..1081820 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
K7X31_RS05115 (1081830) 1081830..1082372 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
K7X31_RS05120 (1082372) 1082372..1083139 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
K7X31_RS05125 (1083136) 1083136..1083546 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
K7X31_RS05130 (1083539) 1083539..1084009 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
K7X31_RS05135 (1084176) 1084176..1084487 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
K7X31_RS05140 (1084484) 1084484..1084903 + 420 WP_001600394.1 helix-turn-helix domain-containing protein Antitoxin
K7X31_RS05145 (1084982) 1084982..1086406 - 1425 WP_223024956.1 6-phospho-beta-glucosidase AscB -
K7X31_RS05150 (1086415) 1086415..1087872 - 1458 WP_001107837.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
K7X31_RS05155 (1088132) 1088132..1089142 + 1011 WP_024180340.1 DNA-binding transcriptional regulator AscG -
K7X31_RS05160 (1089291) 1089291..1089818 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T214698 WP_000547555.1 NZ_CP082357:1084176-1084487 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T214698 NZ_CP110190:c5772524-5772246 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15389.40 Da        Isoelectric Point: 4.4596

>AT214698 WP_001600394.1 NZ_CP082357:1084484-1084903 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYESNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT214698 NZ_CP110190:c5772234-5771929 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2G9D6

References