Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 621484..622283 Replicon chromosome
Accession NZ_CP082357
Organism Escherichia coli strain VCSW7

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag K7X31_RS02945 Protein ID WP_000347273.1
Coordinates 621484..621948 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K7X31_RS02950 Protein ID WP_001307405.1
Coordinates 621948..622283 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7X31_RS02915 (616485) 616485..616919 - 435 WP_089703953.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K7X31_RS02920 (616937) 616937..617815 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K7X31_RS02925 (617805) 617805..618584 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K7X31_RS02930 (618595) 618595..619068 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K7X31_RS02935 (619091) 619091..620371 - 1281 WP_223024888.1 tagatose-bisphosphate aldolase subunit KbaZ -
K7X31_RS02940 (620620) 620620..621429 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
K7X31_RS02945 (621484) 621484..621948 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K7X31_RS02950 (621948) 621948..622283 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K7X31_RS02955 (622432) 622432..624003 - 1572 WP_001273756.1 galactarate dehydratase -
K7X31_RS02960 (624378) 624378..625712 + 1335 WP_000599654.1 galactarate/glucarate/glycerate transporter GarP -
K7X31_RS02965 (625728) 625728..626498 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T214695 WP_000347273.1 NZ_CP082357:c621948-621484 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T214695 NZ_CP110190:c5454437-5454138 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT214695 WP_001307405.1 NZ_CP082357:c622283-621948 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT214695 NZ_CP110190:c5454141-5453851 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References