Detailed information of TA system TA214692

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39717..39986
• Replicon: plasmid pVCSW5
• Accession: NZ_CP082356
• Organism: Escherichia coli strain VCSW5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K7X33_RS22875
• Protein ID: WP_001372321.1
• Coordinates: 39861..39986 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39717..39782 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X33_RS23275	34761..35012	+	252	WP_071828952.1	hypothetical protein	-
K7X33_RS23280	35253..35459	+	207	WP_000547968.1	hypothetical protein	-
K7X33_RS22845	35485..36024	+	540	WP_105633039.1	single-stranded DNA-binding protein	-
K7X33_RS22850	36086..36319	+	234	WP_105633040.1	DUF905 family protein	-
K7X33_RS22855	36385..38343	+	1959	WP_105633041.1	ParB/RepB/Spo0J family partition protein	-
K7X33_RS22860	38398..38832	+	435	WP_105633042.1	conjugation system SOS inhibitor PsiB	-
K7X33_RS22865	38829..39591	+	763	Protein_44	plasmid SOS inhibition protein A	-
K7X33_RS22870	39560..39748	-	189	WP_001299721.1	hypothetical protein	-
-	39560..39784	+	225	NuclAT_0	-	-
-	39560..39784	+	225	NuclAT_0	-	-
-	39560..39784	+	225	NuclAT_0	-	-
-	39560..39784	+	225	NuclAT_0	-	-
-	39717..39782	-	66	-	-	Antitoxin
K7X33_RS23285	39770..39919	+	150	Protein_46	plasmid maintenance protein Mok	-
K7X33_RS22875	39861..39986	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
K7X33_RS23290	40207..40437	+	231	WP_001426396.1	hypothetical protein	-
K7X33_RS23295	40435..40608	-	174	Protein_49	hypothetical protein	-
K7X33_RS23300	40678..40884	+	207	WP_000547968.1	hypothetical protein	-
K7X33_RS22880	40909..41196	+	288	WP_000107535.1	hypothetical protein	-
K7X33_RS22885	41280..42476	-	1197	WP_001358663.1	IS110-like element ISEc20 family transposase	-
K7X33_RS22890	42769..43590	+	822	WP_143189711.1	DUF932 domain-containing protein	-
K7X33_RS22895	43887..44477	-	591	WP_165850686.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeC / faeD / faeE / faeF / faeH / faeI / faeJ	1..88468	88468
	-	flank	IS/Tn	-	-	41280..42476	1196

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T214692 WP_001372321.1 NZ_CP082356:39861-39986 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T214692 NZ_CP110190:c4563425-4562670 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCAGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGGCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT214692 NZ_CP082356:c39782-39717 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin