Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4569002..4569604 Replicon chromosome
Accession NZ_CP082355
Organism Escherichia coli strain VCSW5

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag K7X33_RS21735 Protein ID WP_000897305.1
Coordinates 4569293..4569604 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K7X33_RS21730 Protein ID WP_000356400.1
Coordinates 4569002..4569292 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7X33_RS21700 (4564784) 4564784..4565686 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
K7X33_RS21705 (4565683) 4565683..4566318 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
K7X33_RS21710 (4566315) 4566315..4567244 + 930 WP_000027699.1 formate dehydrogenase accessory protein FdhE -
K7X33_RS21715 (4567281) 4567281..4567511 - 231 WP_001309107.1 CopG family transcriptional regulator -
K7X33_RS21720 (4567728) 4567728..4567946 - 219 WP_001295676.1 CopG family transcriptional regulator -
K7X33_RS21725 (4568365) 4568365..4568643 - 279 WP_001306650.1 hypothetical protein -
K7X33_RS21730 (4569002) 4569002..4569292 - 291 WP_000356400.1 NadS family protein Antitoxin
K7X33_RS21735 (4569293) 4569293..4569604 - 312 WP_000897305.1 hypothetical protein Toxin
K7X33_RS21740 (4569833) 4569833..4570741 + 909 WP_001306651.1 alpha/beta hydrolase -
K7X33_RS23250 (4570910) 4570910..4571824 - 915 WP_225102955.1 transposase -
K7X33_RS23255 (4571837) 4571837..4572724 - 888 Protein_4251 hypothetical protein -
K7X33_RS21750 (4573140) 4573140..4574081 - 942 WP_024180368.1 fatty acid biosynthesis protein FabY -
K7X33_RS21755 (4574126) 4574126..4574563 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T214690 WP_000897305.1 NZ_CP082355:c4569604-4569293 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T214690 NZ_CP110190:1826458-1827045 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10860.45 Da        Isoelectric Point: 10.8907

>AT214690 WP_000356400.1 NZ_CP082355:c4569292-4569002 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT214690 NZ_CP110190:1827112-1827966 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAACAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGTTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAAACCCAGACCCGCTGGCGCGAGACGATGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGCGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGACAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGGGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGACATACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References