Detailed information of TA system TA214673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1082840..1083567
• Replicon: chromosome
• Accession: NZ_CP082355
• Organism: Escherichia coli strain VCSW5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D6JE58
• Locus tag: K7X33_RS05145
• Protein ID: WP_000547557.1
• Coordinates: 1082840..1083151 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K7X33_RS05150
• Protein ID: WP_000126294.1
• Coordinates: 1083148..1083567 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X33_RS05120 (1078775)	1078775..1080484	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
K7X33_RS05125 (1080494)	1080494..1081036	+	543	WP_000493779.1	formate hydrogenlyase subunit HycF	-
K7X33_RS05130 (1081036)	1081036..1081803	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
K7X33_RS05135 (1081800)	1081800..1082210	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
K7X33_RS05140 (1082203)	1082203..1082673	+	471	WP_000132947.1	hydrogenase maturation peptidase HycI	-
K7X33_RS05145 (1082840)	1082840..1083151	+	312	WP_000547557.1	type II toxin-antitoxin system HigB family toxin	Toxin
K7X33_RS05150 (1083148)	1083148..1083567	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
K7X33_RS05155 (1083647)	1083647..1085071	-	1425	WP_000110380.1	6-phospho-beta-glucosidase AscB	-
K7X33_RS05160 (1085080)	1085080..1086537	-	1458	WP_001107837.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
K7X33_RS05165 (1086797)	1086797..1087807	+	1011	WP_024180340.1	DNA-binding transcriptional regulator AscG	-
K7X33_RS05170 (1087956)	1087956..1088483	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12467.20 Da
• Isoelectric Point: 9.5262

>T214673 WP_000547557.1 NZ_CP082355:1082840-1083151 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T214673 NZ_CP110170:4004329-4004547 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT214673 WP_000126294.1 NZ_CP082355:1083148-1083567 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT214673 NZ_CP110170:4003928-4004302 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: D6JE58