Detailed information of TA system TA214670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 619871..620670
• Replicon: chromosome
• Accession: NZ_CP082355
• Organism: Escherichia coli strain VCSW5

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: K7X33_RS02960
• Protein ID: WP_000347273.1
• Coordinates: 619871..620335 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: K7X33_RS02965
• Protein ID: WP_001308975.1
• Coordinates: 620335..620670 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X33_RS02930 (614872)	614872..615306	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
K7X33_RS02935 (615324)	615324..616202	-	879	WP_001308976.1	PTS N-acetylgalactosamine transporter subunit IID	-
K7X33_RS02940 (616192)	616192..616971	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
K7X33_RS02945 (616982)	616982..617455	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
K7X33_RS02950 (617478)	617478..618758	-	1281	WP_000681954.1	tagatose-bisphosphate aldolase subunit KbaZ	-
K7X33_RS02955 (619007)	619007..619816	+	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
K7X33_RS02960 (619871)	619871..620335	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
K7X33_RS02965 (620335)	620335..620670	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
K7X33_RS02970 (620819)	620819..622390	-	1572	WP_001273756.1	galactarate dehydratase	-
K7X33_RS02975 (622765)	622765..624099	+	1335	WP_000599654.1	galactarate/glucarate/glycerate transporter GarP	-
K7X33_RS02980 (624115)	624115..624885	+	771	WP_001058238.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T214670 WP_000347273.1 NZ_CP082355:c620335-619871 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T214670 NZ_CP110170:839201-839590 [Klebsiella pneumoniae]
ATGCAAACCAAATCATCCCCCCTCATGCGGGCGGCATCGTCACATCCGTCTCCCGTGGGTGTCTGGCAGACTCTGCTGAC
CAGCCTGCTTGAACACCACTATGGCCTCACGCTTAGCGATACGCCGTTCAGCGATGAACAGGTGATTCAGCAGCATATCG
AGGCGGGTATCTCGCTGGCCGATGCCCTGAATTTTATTGTTGAAAAGTATGAACTGGTCCGGACTGACCGGCAAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGAAAGGCTACAGGGCTGATGAACCTTGG
CACGTATAAAGACGTGACGGCTATTACCAAAGGACAACATCAGCAGGCCAACACTTCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT214670 WP_001308975.1 NZ_CP082355:c620670-620335 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT214670 NZ_CP110170:838786-839145 [Klebsiella pneumoniae]
TTGTCGAAGAAAACCACGATAACAACGCATGATATCAGTGAACCCTGGTGGGGGCTCAGGCGCAGTGTCTCCCCGTGTTT
TGGTGCCCGTCTGGTCCAGGAGGGAAACCGCCTGCATTACCTGGCTGACCGGGCGAACTTTGACTGTCAGCCAGTTAATG
CGGATTTGCGCCATCTTGACCAGGCTTTCCCGGTACTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAAT
CCCCGTCATCAGCACTGCGTCAAGTTGTATGCAAAAGGTCTGACCTGCGAGGCCGATACGCTCGGTTCGCACGGCTATGT
TTATATCGCGATTTATCCAACTCCCGTCGCCACGGCATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08