Detailed information of TA system TA214667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 40719..41251
• Replicon: plasmid pVCSW10.3
• Accession: NZ_CP082354
• Organism: Providencia rettgeri strain VCSW10

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: K7H99_RS22045
• Protein ID: WP_222961202.1
• Coordinates: 40976..41251 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: K7H99_RS22040
• Protein ID: WP_282565801.1
• Coordinates: 40719..40982 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H99_RS22010 (K7H99_22010)	35817..36107	-	291	WP_222961194.1	hypothetical protein	-
K7H99_RS22365	36265..36387	+	123	WP_262417065.1	hypothetical protein	-
K7H99_RS22015 (K7H99_22015)	36779..38047	-	1269	WP_222961195.1	translesion error-prone DNA polymerase V subunit UmuC	-
K7H99_RS22020 (K7H99_22020)	38056..38436	-	381	Protein_44	translesion error-prone DNA polymerase V autoproteolytic subunit	-
K7H99_RS22030 (K7H99_22030)	38866..39582	-	717	WP_282560778.1	ParA family protein	-
K7H99_RS22035 (K7H99_22035)	39916..40545	+	630	WP_222961199.1	recombinase family protein	-
K7H99_RS22040 (K7H99_22040)	40719..40982	+	264	WP_282565801.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
K7H99_RS22045 (K7H99_22045)	40976..41251	+	276	WP_222961202.1	type II toxin-antitoxin system YafQ family toxin	Toxin
K7H99_RS22050 (K7H99_22050)	41459..41851	+	393	WP_222961204.1	hypothetical protein	-
K7H99_RS22055 (K7H99_22055)	42042..42608	-	567	WP_262417066.1	hypothetical protein	-
K7H99_RS22060 (K7H99_22060)	42728..43348	-	621	WP_222961206.1	DUF2913 family protein	-
K7H99_RS22065 (K7H99_22065)	43294..43914	-	621	WP_282565802.1	ProQ/FINO family protein	-
K7H99_RS22070 (K7H99_22070)	44617..45567	-	951	WP_282565803.1	RepA family replication protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..51921	51921

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (7-88)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-78)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10607.27 Da
• Isoelectric Point: 10.1555

>T214667 WP_222961202.1 NZ_CP082354:40976-41251 [Providencia rettgeri]
MLTPIRSSAFKADVKRQKKRGKDLNKLKVLIELLIKQEQIPADYVDHPLTGNWRGYRDAHIEGDWLLIYKITGQELKLAR
TGSHQDIFSQY

Nucleotide

• Length: 276 bp

>T214667 NZ_CP110169:c6177-5872 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9380.88 Da
• Isoelectric Point: 5.8349

>AT214667 WP_282565801.1 NZ_CP082354:40719-40982 [Providencia rettgeri]
MISDTTVKARISHDVKERAVSALAKMGLNTSDLIRMVMIRVADEGKLPFEIKTPNVVTKEAIEELEKGNGKAFSSTNALF
DDLGIC

Nucleotide

• Length: 264 bp

>AT214667 NZ_CP110169:c6397-6179 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA