Detailed information of TA system TA214659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4522393..4522995
• Replicon: chromosome
• Accession: NZ_CP082349
• Organism: Escherichia coli strain AL.H8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: K7H97_RS21945
• Protein ID: WP_000897305.1
• Coordinates: 4522684..4522995 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K7H97_RS21940
• Protein ID: WP_000356397.1
• Coordinates: 4522393..4522683 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H97_RS21915 (4518319)	4518319..4519221	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
K7H97_RS21920 (4519218)	4519218..4519853	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
K7H97_RS21925 (4519850)	4519850..4520779	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
K7H97_RS21930 (4521109)	4521109..4521351	-	243	WP_001087409.1	protein YiiF	-
K7H97_RS21935 (4521570)	4521570..4521788	-	219	WP_001295676.1	CopG family transcriptional regulator	-
K7H97_RS21940 (4522393)	4522393..4522683	-	291	WP_000356397.1	NadS family protein	Antitoxin
K7H97_RS21945 (4522684)	4522684..4522995	-	312	WP_000897305.1	hypothetical protein	Toxin
K7H97_RS21950 (4523224)	4523224..4524132	+	909	WP_001299484.1	alpha/beta hydrolase	-
K7H97_RS21955 (4524196)	4524196..4525137	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
K7H97_RS21960 (4525182)	4525182..4525619	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
K7H97_RS21965 (4525616)	4525616..4526485	-	870	WP_032318972.1	virulence factor BrkB family protein	-
K7H97_RS21970 (4526479)	4526479..4527078	-	600	WP_001295269.1	glucose-1-phosphatase	-
K7H97_RS21975 (4527177)	4527177..4527962	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T214659 WP_000897305.1 NZ_CP082349:c4522995-4522684 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T214659 NZ_CP110167:4051010-4051228 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT214659 WP_000356397.1 NZ_CP082349:c4522683-4522393 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT214659 NZ_CP110167:4050609-4050983 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7