Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3718049..3718590 Replicon chromosome
Accession NZ_CP082349
Organism Escherichia coli strain AL.H8

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag K7H97_RS18210 Protein ID WP_000615979.1
Coordinates 3718312..3718590 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag K7H97_RS18205 Protein ID WP_000729704.1
Coordinates 3718049..3718309 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7H97_RS18160 (3713253) 3713253..3713684 - 432 WP_024228049.1 flagellar basal body rod protein FlgC -
K7H97_RS18165 (3713687) 3713687..3714022 - 336 WP_000512950.1 flagellar biosynthesis protein FlgB -
K7H97_RS18170 (3714104) 3714104..3714841 + 738 WP_174561621.1 flagellar basal body P-ring formation chaperone FlgA -
K7H97_RS18175 (3714922) 3714922..3715200 + 279 WP_174561622.1 flagellar biosynthesis anti-sigma factor FlgM -
K7H97_RS18180 (3715213) 3715213..3715638 + 426 Protein_3564 flagellar protein FlgN -
K7H97_RS18185 (3715639) 3715639..3716301 + 663 Protein_3565 flagellar biosynthesis protein FlhA -
K7H97_RS18190 (3716406) 3716406..3716684 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
K7H97_RS18195 (3716677) 3716677..3717033 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
K7H97_RS18200 (3717090) 3717090..3717863 - 774 WP_134894404.1 C40 family peptidase -
K7H97_RS18205 (3718049) 3718049..3718309 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K7H97_RS18210 (3718312) 3718312..3718590 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K7H97_RS18215 (3718746) 3718746..3719486 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K7H97_RS18220 (3719457) 3719457..3720224 - 768 WP_001365365.1 class II glutamine amidotransferase -
K7H97_RS18225 (3720430) 3720430..3721008 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T214656 WP_000615979.1 NZ_CP082349:3718312-3718590 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T214656 NZ_CP110167:806790-807200 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT214656 WP_000729704.1 NZ_CP082349:3718049-3718309 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT214656 NZ_CP110167:806543-806809 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References