Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 610161..610960 Replicon chromosome
Accession NZ_CP082349
Organism Escherichia coli strain AL.H8

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag K7H97_RS02980 Protein ID WP_000347273.1
Coordinates 610161..610625 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K7H97_RS02985 Protein ID WP_001307405.1
Coordinates 610625..610960 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7H97_RS02950 (605162) 605162..605596 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K7H97_RS02955 (605614) 605614..606492 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K7H97_RS02960 (606482) 606482..607261 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K7H97_RS02965 (607272) 607272..607745 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K7H97_RS02970 (607768) 607768..609048 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
K7H97_RS02975 (609297) 609297..610106 + 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
K7H97_RS02980 (610161) 610161..610625 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K7H97_RS02985 (610625) 610625..610960 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K7H97_RS02990 (611109) 611109..612680 - 1572 WP_001273741.1 galactarate dehydratase -
K7H97_RS02995 (613055) 613055..614389 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
K7H97_RS03000 (614405) 614405..615175 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 610161..621948 11787


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T214637 WP_000347273.1 NZ_CP082349:c610625-610161 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T214637 NZ_CP110156:3174-3455 [Klebsiella pneumoniae]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT214637 WP_001307405.1 NZ_CP082349:c610960-610625 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT214637 NZ_CP110156:2933-3184 [Klebsiella pneumoniae]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References