Detailed information of TA system TA214633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 39346..40001
• Replicon: plasmid pLsch
• Accession: NZ_CP082348
• Organism: Pantoea dispersa strain Lsch

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K7H94_RS22280
• Protein ID: WP_125311853.1
• Coordinates: 39642..40001 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A246NG11
• Locus tag: K7H94_RS22275
• Protein ID: WP_059012908.1
• Coordinates: 39346..39645 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H94_RS22245 (K7H94_22245)	34968..35171	-	204	WP_245001442.1	hypothetical protein	-
K7H94_RS22250 (K7H94_22250)	35305..36344	+	1040	Protein_51	translesion error-prone DNA polymerase V subunit UmuC	-
K7H94_RS22255 (K7H94_22255)	36660..36899	-	240	WP_059013031.1	hypothetical protein	-
K7H94_RS22260 (K7H94_22260)	36983..37216	-	234	WP_222952539.1	plasmid partition protein ParG	-
K7H94_RS22270 (K7H94_22270)	38443..39150	+	708	Protein_55	DUF4113 domain-containing protein	-
K7H94_RS22275 (K7H94_22275)	39346..39645	-	300	WP_059012908.1	XRE family transcriptional regulator	Antitoxin
K7H94_RS22280 (K7H94_22280)	39642..40001	-	360	WP_125311853.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K7H94_RS23090	40232..40681	-	450	WP_245001443.1	hypothetical protein	-
K7H94_RS22290 (K7H94_22290)	40731..42032	+	1302	WP_222952507.1	hypothetical protein	-
K7H94_RS22295 (K7H94_22295)	42029..42667	+	639	WP_088516990.1	LysE family transporter	-
K7H94_RS22300 (K7H94_22300)	42638..43456	-	819	WP_059012900.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..181041	181041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-87)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13808.98 Da
• Isoelectric Point: 10.0345

>T214633 WP_125311853.1 NZ_CP082348:c40001-39642 [Pantoea dispersa]
VWLVETTDLFAEWLKEQDRAMQLDVLAALNLLKQEGPHLGRPHVDTLYGSKIPNMKELRVQSNGRPVRGFFVFDPMRKAI
VLCAGNKEGADEKRFYKTMIKTAENEYHRHLDRLKRSGK

Nucleotide

• Length: 360 bp

>T214633 NZ_CP110154:22095-22406 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCATTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTAGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10824.46 Da
• Isoelectric Point: 4.8430

>AT214633 WP_059012908.1 NZ_CP082348:c39645-39346 [Pantoea dispersa]
MKTLDDLMADLTPEELTQVHEKVAAMRLDSQLYKLREALSKTQKQQAEAMGIAQPSVAAIEGRGEDLKIATLKRYVEALG
GQLTIGVNMPGGEHVDFTL

Nucleotide

• Length: 300 bp

>AT214633 NZ_CP110154:22403-22822 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A246NG11