Detailed information of TA system TA214631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 568834..569488
• Replicon: plasmid unnamed
• Accession: NZ_CP082347
• Organism: Pantoea dispersa strain Lsch

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K7H94_RS21345
• Protein ID: WP_222952177.1
• Coordinates: 568834..569205 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A246NDM6
• Locus tag: K7H94_RS21350
• Protein ID: WP_021508926.1
• Coordinates: 569189..569488 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H94_RS21325 (K7H94_21325)	564117..565496	+	1380	WP_222952176.1	heavy metal sensor histidine kinase	-
K7H94_RS21330 (K7H94_21330)	565526..566179	-	654	WP_058770319.1	oxygen-insensitive NAD(P)H nitroreductase	-
K7H94_RS21335 (K7H94_21335)	566284..567285	-	1002	WP_021508931.1	zinc-binding alcohol dehydrogenase family protein	-
K7H94_RS21340 (K7H94_21340)	567422..568339	+	918	WP_058759023.1	LysR family transcriptional regulator	-
K7H94_RS21345 (K7H94_21345)	568834..569205	+	372	WP_222952177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K7H94_RS21350 (K7H94_21350)	569189..569488	+	300	WP_021508926.1	XRE family transcriptional regulator	Antitoxin
K7H94_RS21355 (K7H94_21355)	569566..570033	-	468	WP_058759022.1	hypothetical protein	-
K7H94_RS21360 (K7H94_21360)	570219..571148	-	930	WP_058781069.1	ABC transporter substrate-binding protein	-
K7H94_RS21365 (K7H94_21365)	571158..571892	-	735	WP_222952178.1	ABC transporter permease	-
K7H94_RS21370 (K7H94_21370)	571876..572592	-	717	WP_222952179.1	ABC transporter ATP-binding protein	-
K7H94_RS21375 (K7H94_21375)	572589..573557	-	969	WP_222952180.1	HesA/MoeB/ThiF family protein	-
K7H94_RS21380 (K7H94_21380)	573568..574326	-	759	WP_222952181.1	thiazole synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..689940	689940

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (10-88)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14405.59 Da
• Isoelectric Point: 10.3096

>T214631 WP_222952177.1 NZ_CP082347:568834-569205 [Pantoea dispersa]
VWQVEATDRFWKWLQTQDEALRLDVLAAMKLLAQDGPHLGRPFVDTLMLSRVPNMKELRVQSKGRPIRSFFVFDPRRHAI
VLCAGNKQGKNQKRFYQQMLRIAETEYHHHLNAIGETHNENLG

Nucleotide

• Length: 372 bp

>T214631 NZ_CP110153:c5380238-5379855 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11265.00 Da
• Isoelectric Point: 6.7255

>AT214631 WP_021508926.1 NZ_CP082347:569189-569488 [Pantoea dispersa]
MKTLDEMLAALTPEQQHQVDERVSQMRLEYQLYQLREQMQRTQRQQAQAMHIAQSSVAAMEARGAELKISTLKRYIEALG
GELTLGVKMPGGQHVDLII

Nucleotide

• Length: 300 bp

>AT214631 NZ_CP110153:c5380480-5380238 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A246NDM6