Detailed information of TA system TA214625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3504419..3505004
• Replicon: chromosome
• Accession: NZ_CP082346
• Organism: Pantoea dispersa strain Lsch

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3TYM8
• Locus tag: K7H94_RS23035
• Protein ID: WP_010615566.1
• Coordinates: 3504705..3505004 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A246NKX1
• Locus tag: K7H94_RS16440
• Protein ID: WP_010615565.1
• Coordinates: 3504419..3504718 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H94_RS16420 (K7H94_16420)	3500420..3501721	+	1302	WP_058759604.1	anaerobic C4-dicarboxylate transporter	-
K7H94_RS16425 (K7H94_16425)	3501808..3503505	+	1698	WP_222951351.1	protein-disulfide reductase DsbD	-
K7H94_RS16430 (K7H94_16430)	3503572..3504150	+	579	WP_021507771.1	transcriptional regulator	-
K7H94_RS16440 (K7H94_16440)	3504419..3504718	-	300	WP_010615565.1	helix-turn-helix transcriptional regulator	Antitoxin
K7H94_RS23035	3504705..3505004	-	300	WP_010615566.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K7H94_RS16450 (K7H94_16450)	3505476..3505724	+	249	Protein_3213	LysR family transcriptional regulator	-
K7H94_RS16455 (K7H94_16455)	3505825..3506994	-	1170	WP_222951352.1	Gfo/Idh/MocA family oxidoreductase	-
K7H94_RS16460 (K7H94_16460)	3507005..3507928	-	924	WP_058759607.1	TIM barrel protein	-
K7H94_RS16465 (K7H94_16465)	3507927..3508097	+	171	WP_222951353.1	hypothetical protein	-
K7H94_RS16470 (K7H94_16470)	3508127..3509146	-	1020	WP_222951354.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (4-87)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-88)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11308.01 Da
• Isoelectric Point: 10.2176

>T214625 WP_010615566.1 NZ_CP082346:c3505004-3504705 [Pantoea dispersa]
MIEDVLASLAVLREFGPTLGRPDVDTLVGSRFSNMKELRVQSNGRAIRAFFAFDPVRRAIVLCAGNKTGTHQRRFYQAMI
KLADREYQQHLEEMNHAKT

Nucleotide

• Length: 300 bp

>T214625 NZ_CP110153:c2714064-2712742 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCACCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACTCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10921.75 Da
• Isoelectric Point: 9.7643

>AT214625 WP_010615565.1 NZ_CP082346:c3504718-3504419 [Pantoea dispersa]
MPKLEDLIAQRSPESQARIRNKAAEKILEIRLRQLREELALSQVQLAEKMGVSQPAIAAIEARGDELKLSSLKKYVEALG
GKLSVTVELPAGDGRIFQL

Nucleotide

• Length: 300 bp

>AT214625 NZ_CP110153:c2714330-2714064 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGATGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A246NKX1