Detailed information of TA system TA214621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5768..6423
• Replicon: chromosome
• Accession: NZ_CP082346
• Organism: Pantoea dispersa strain Lsch

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K7H94_RS00030
• Protein ID: WP_058758198.1
• Coordinates: 6070..6423 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A246NK47
• Locus tag: K7H94_RS00025
• Protein ID: WP_058758199.1
• Coordinates: 5768..6073 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H94_RS00010 (K7H94_00010)	942..2042	+	1101	WP_010617831.1	DNA polymerase III subunit beta	-
K7H94_RS00015 (K7H94_00015)	2214..3299	+	1086	WP_222951463.1	DNA replication/repair protein RecF	-
K7H94_RS00020 (K7H94_00020)	3317..5725	+	2409	WP_058758200.1	DNA topoisomerase (ATP-hydrolyzing) subunit B	-
K7H94_RS00025 (K7H94_00025)	5768..6073	-	306	WP_058758199.1	helix-turn-helix transcriptional regulator	Antitoxin
K7H94_RS00030 (K7H94_00030)	6070..6423	-	354	WP_058758198.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K7H94_RS00035 (K7H94_00035)	6630..7439	+	810	WP_058758197.1	sugar-phosphatase	-
K7H94_RS00040 (K7H94_00040)	7655..8353	+	699	WP_021506909.1	FadR/GntR family transcriptional regulator	-
K7H94_RS00045 (K7H94_00045)	8350..9228	+	879	WP_222951464.1	2-dehydro-3-deoxygalactonokinase	-
K7H94_RS00050 (K7H94_00050)	9212..9829	+	618	WP_021506907.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
K7H94_RS00055 (K7H94_00055)	9826..10974	+	1149	WP_021506906.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (11-88)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13609.64 Da
• Isoelectric Point: 9.6733

>T214621 WP_058758198.1 NZ_CP082346:c6423-6070 [Pantoea dispersa]
MWEIIFTDTFREWLRAQDNKLKKRIAAALLNLQHYGPLLPRPYADTVNGSRFHNMKELRIQYAGQPIRALYAFDTQRKAV
ILCAGNKSGDKQFYARMICLADQLFTAYLNNAEENHS

Nucleotide

• Length: 354 bp

>T214621 NZ_CP110153:337424-337792 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11273.02 Da
• Isoelectric Point: 4.3253

>AT214621 WP_058758199.1 NZ_CP082346:c6073-5768 [Pantoea dispersa]
MNDLQKFIESEFTEAEQADIQLMADELILETGLQLLREELALSQRTVAEAMGVKQPRITQIEQGGADQRLITIKRYIEAM
GGKLSLLIEMPDGSGGRILRV

Nucleotide

• Length: 306 bp

>AT214621 NZ_CP110153:337206-337427 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A246NK47