Detailed information of TA system TA214608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2095526..2096175
• Replicon: chromosome
• Accession: NZ_CP082341
• Organism: Pantoea dispersa strain AHKW2b

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K7X52_RS09725
• Protein ID: WP_088517162.1
• Coordinates: 2095825..2096175 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A246NEP9
• Locus tag: K7X52_RS09720
• Protein ID: WP_021505978.1
• Coordinates: 2095526..2095825 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X52_RS09700 (K7X52_09700)	2091047..2091802	+	756	WP_021505974.1	pyrroloquinoline-quinone synthase PqqC	-
K7X52_RS09705 (K7X52_09705)	2091807..2092085	+	279	WP_021505975.1	pyrroloquinoline quinone biosynthesis peptide chaperone PqqD	-
K7X52_RS09710 (K7X52_09710)	2092072..2093214	+	1143	WP_058758602.1	pyrroloquinoline quinone biosynthesis protein PqqE	-
K7X52_RS09715 (K7X52_09715)	2093214..2095535	+	2322	WP_166718983.1	pyrroloquinoline quinone biosynthesis protein PqqF	-
K7X52_RS09720 (K7X52_09720)	2095526..2095825	-	300	WP_021505978.1	helix-turn-helix transcriptional regulator	Antitoxin
K7X52_RS09725 (K7X52_09725)	2095825..2096175	-	351	WP_088517162.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K7X52_RS09730 (K7X52_09730)	2096612..2096797	-	186	WP_222986646.1	hypothetical protein	-
K7X52_RS09735 (K7X52_09735)	2096832..2098835	+	2004	WP_222986647.1	protein-disulfide reductase DsbD family protein	-
K7X52_RS09740 (K7X52_09740)	2098835..2099557	+	723	WP_088517198.1	DsbA family protein	-
K7X52_RS09745 (K7X52_09745)	2099554..2100057	+	504	WP_215480330.1	thioredoxin domain-containing protein	-
K7X52_RS09750 (K7X52_09750)	2100317..2101153	+	837	WP_222986648.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13370.60 Da
• Isoelectric Point: 7.7777

>T214608 WP_088517162.1 NZ_CP082341:c2096175-2095825 [Pantoea dispersa]
MWEILTTDVFDDWFLAQNEGLREDVLAVMQILAEMGPKPGRPYVDTLKGCQFPNLKELRVQHAGMPVRAFFAFDPVRRAI
VLCAGNKAGCQQKQFYRTMIRLAEAEYRKHLVHMEG

Nucleotide

• Length: 351 bp

>T214608 NZ_CP110150:729557-730042 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11201.90 Da
• Isoelectric Point: 10.0004

>AT214608 WP_021505978.1 NZ_CP082341:c2095825-2095526 [Pantoea dispersa]
MGTYKQLLAKQSPESQQRIAEKVEKLRQEIALSQLREELNLSQTELARTIGVSQPTLAKMENPENDPRLSTLKRYVAALG
GELRIDVKLPNGKHFAFQL

Nucleotide

• Length: 300 bp

>AT214608 NZ_CP110150:729267-729560 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A246NEP9