Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 166523..167203 Replicon chromosome
Accession NZ_CP082341
Organism Pantoea dispersa strain AHKW2b

Toxin (Protein)


Gene name higB Uniprot ID A0A246NHY2
Locus tag K7X52_RS00780 Protein ID WP_059013049.1
Coordinates 166523..166822 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A246NHX0
Locus tag K7X52_RS00785 Protein ID WP_058775157.1
Coordinates 166871..167203 (+) Length 111 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7X52_RS00755 (K7X52_00755) 161822..162316 + 495 WP_021507883.1 GNAT family N-acetyltransferase -
K7X52_RS00760 (K7X52_00760) 162423..163253 + 831 WP_088516542.1 hypothetical protein -
K7X52_RS00765 (K7X52_00765) 163255..163578 + 324 WP_058759357.1 contact-dependent growth inhibition system immunity protein -
K7X52_RS00770 (K7X52_00770) 163719..164633 - 915 WP_021507882.1 sugar ABC transporter substrate-binding protein -
K7X52_RS00775 (K7X52_00775) 165069..166463 + 1395 WP_222986017.1 MFS transporter -
K7X52_RS00780 (K7X52_00780) 166523..166822 + 300 WP_059013049.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K7X52_RS00785 (K7X52_00785) 166871..167203 + 333 WP_058775157.1 HigA family addiction module antitoxin Antitoxin
K7X52_RS00790 (K7X52_00790) 167295..168458 + 1164 WP_222986018.1 HD-GYP domain-containing protein -
K7X52_RS00795 (K7X52_00795) 168567..168656 + 90 WP_021507879.1 K(+)-transporting ATPase subunit F -
K7X52_RS00800 (K7X52_00800) 168656..170335 + 1680 WP_104094188.1 potassium-transporting ATPase subunit KdpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(6-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11503.22 Da        Isoelectric Point: 10.4912

>T214606 WP_059013049.1 NZ_CP082341:166523-166822 [Pantoea dispersa]
MGSIRAFRQAWLAQFYTHGTPHAQIPRAIESALARKLDIIHAATSHQDLRSPPGNRFEALKPPLLGYYSIRVNAQYRLIF
QWAKGEAWDLYLDPHRYKK

Download         Length: 300 bp

>T214606 NZ_CP110150:324656-325024 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 111 a.a.        Molecular weight: 12751.58 Da        Isoelectric Point: 7.2039

>AT214606 WP_058775157.1 NZ_CP082341:166871-167203 [Pantoea dispersa]
MKQAMRKPTSVGDVLQYEFLEPLNLKVNDLAEMLNVHRNTVSALVNNNRKLSTEMAYRLARVFATSIEFWLNLQRHLDQW
EVENDARTQEELSRVMTLSAFLAQRGTQVA

Download         Length: 333 bp

>AT214606 NZ_CP110150:324438-324659 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGCTCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A246NHY2

Antitoxin

Source ID Structure
AlphaFold DB A0A246NHX0

References