Detailed information of TA system TA214605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 6236..6891
• Replicon: chromosome
• Accession: NZ_CP082341
• Organism: Pantoea dispersa strain AHKW2b

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K7X52_RS00030
• Protein ID: WP_088516156.1
• Coordinates: 6538..6891 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A246NK47
• Locus tag: K7X52_RS00025
• Protein ID: WP_058758199.1
• Coordinates: 6236..6541 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X52_RS00010 (K7X52_00010)	1410..2510	+	1101	WP_010617831.1	DNA polymerase III subunit beta	-
K7X52_RS00015 (K7X52_00015)	2682..3767	+	1086	WP_058758201.1	DNA replication/repair protein RecF	-
K7X52_RS00020 (K7X52_00020)	3785..6193	+	2409	WP_058758200.1	DNA topoisomerase (ATP-hydrolyzing) subunit B	-
K7X52_RS00025 (K7X52_00025)	6236..6541	-	306	WP_058758199.1	helix-turn-helix transcriptional regulator	Antitoxin
K7X52_RS00030 (K7X52_00030)	6538..6891	-	354	WP_088516156.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K7X52_RS00035 (K7X52_00035)	7098..7907	+	810	WP_058758197.1	sugar-phosphatase	-
K7X52_RS00040 (K7X52_00040)	8124..8822	+	699	WP_222985979.1	FadR/GntR family transcriptional regulator	-
K7X52_RS00045 (K7X52_00045)	8819..9697	+	879	WP_222985980.1	2-dehydro-3-deoxygalactonokinase	-
K7X52_RS00050 (K7X52_00050)	9681..10298	+	618	WP_021506907.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
K7X52_RS00055 (K7X52_00055)	10295..11443	+	1149	WP_222985981.1	galactonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (11-88)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13595.61 Da
• Isoelectric Point: 9.6733

>T214605 WP_088516156.1 NZ_CP082341:c6891-6538 [Pantoea dispersa]
MWEIIFTDTFREWLRAQDNKLKKRIAAALLNLQHYGPLLPRPYADTVNGSRFHNMKELRIQYAGQPIRALYAFDTQRKAV
ILCAGNKSGDKQFYARMVCLADQLFTAYLNNAEENHS

Nucleotide

• Length: 354 bp

>T214605 NZ_CP110149:288645-288929 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11273.02 Da
• Isoelectric Point: 4.3253

>AT214605 WP_058758199.1 NZ_CP082341:c6541-6236 [Pantoea dispersa]
MNDLQKFIESEFTEAEQADIQLMADELILETGLQLLREELALSQRTVAEAMGVKQPRITQIEQGGADQRLITIKRYIEAM
GGKLSLLIEMPDGSGGRILRV

Nucleotide

• Length: 306 bp

>AT214605 NZ_CP110149:288407-288655 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A246NK47