Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 326172..326758 Replicon chromosome
Accession NZ_CP082290
Organism Klebsiella pneumoniae subsp. pneumoniae strain Kp6

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag K6T93_RS01515 Protein ID WP_002920800.1
Coordinates 326390..326758 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag K6T93_RS01510 Protein ID WP_004174006.1
Coordinates 326172..326393 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6T93_RS01490 (K6T93_01490) 322329..323255 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
K6T93_RS01495 (K6T93_01495) 323252..324529 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
K6T93_RS01500 (K6T93_01500) 324526..325293 + 768 WP_032435160.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
K6T93_RS01505 (K6T93_01505) 325295..326008 + 714 WP_087652577.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
K6T93_RS01510 (K6T93_01510) 326172..326393 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K6T93_RS01515 (K6T93_01515) 326390..326758 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K6T93_RS01520 (K6T93_01520) 327031..328347 + 1317 WP_032435158.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
K6T93_RS01525 (K6T93_01525) 328454..329341 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
K6T93_RS01530 (K6T93_01530) 329338..330183 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
K6T93_RS01535 (K6T93_01535) 330185..331255 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 323252..331992 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T214509 WP_002920800.1 NZ_CP082290:326390-326758 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T214509 NZ_CP110118:c5488-5213 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT214509 WP_004174006.1 NZ_CP082290:326172-326393 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT214509 NZ_CP110118:c5772-5488 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References