Detailed information of TA system TA214482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4184478..4185066
• Replicon: chromosome
• Accession: NZ_CP082270
• Organism: Stenotrophomonas sp. DFS-20110405

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K5L94_RS19170
• Protein ID: WP_053450845.1
• Coordinates: 4184788..4185066 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5L94_RS19165
• Protein ID: WP_006471116.1
• Coordinates: 4184478..4184777 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5L94_RS19145 (K5L94_19190)	4179947..4181134	+	1188	WP_053450842.1	pyridoxal phosphate-dependent aminotransferase	-
K5L94_RS19150 (K5L94_19195)	4181222..4183318	-	2097	WP_053450843.1	prolyl oligopeptidase family serine peptidase	-
K5L94_RS19155 (K5L94_19200)	4183388..4183714	-	327	WP_053450844.1	hypothetical protein	-
K5L94_RS19160 (K5L94_19205)	4183842..4184438	+	597	WP_080374966.1	lipocalin family protein	-
K5L94_RS19165 (K5L94_19210)	4184478..4184777	-	300	WP_006471116.1	HigA family addiction module antitoxin	Antitoxin
K5L94_RS19170 (K5L94_19215)	4184788..4185066	-	279	WP_053450845.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5L94_RS19175 (K5L94_19220)	4185266..4185955	-	690	WP_006471118.1	methionine ABC transporter permease	-
K5L94_RS19180 (K5L94_19225)	4185952..4186959	-	1008	WP_006471119.1	methionine ABC transporter ATP-binding protein	-
K5L94_RS19185 (K5L94_19230)	4187107..4188003	-	897	WP_006471120.1	DMT family transporter	-
K5L94_RS19190 (K5L94_19235)	4188217..4188699	+	483	WP_164241413.1	YajQ family cyclic di-GMP-binding protein	-
K5L94_RS19195 (K5L94_19240)	4188801..4189367	+	567	WP_053451384.1	DUF1415 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (25-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10740.27 Da
• Isoelectric Point: 10.4115

>T214482 WP_053450845.1 NZ_CP082270:c4185066-4184788 [Stenotrophomonas sp. DFS-20110405]
MIRSFRCKQTRALFEGTCPRAWRPIRRVAERKLQLLDSAQTLAFLRSPPGNRLEALSGDRRGQYSLRINDQWRLCFTWTD
SGADAVEIVDYH

Nucleotide

• Length: 279 bp

>T214482 NZ_CP110109:463508-464245 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTATACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCTGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCATCAGTATTCGGTAAGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGCGGAAAAGATTGGTGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10869.59 Da
• Isoelectric Point: 11.3450

>AT214482 WP_006471116.1 NZ_CP082270:c4184777-4184478 [Stenotrophomonas sp. DFS-20110405]
MKRPHNGMRAIHPGEILREEFLLPLALSVNALAKALDVPATRLHAIVHGQRSISADTAARLARHFGGDAASWLAMQAAYD
LKTLPTRSEIERRVQPRAA

Nucleotide

• Length: 300 bp

>AT214482 NZ_CP110109:464358-464486 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA