Detailed information of TA system TA214443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1379024..1379856
• Replicon: chromosome
• Accession: NZ_CP082238
• Organism: Acidithiobacillus ferrooxidans strain BYM

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: B7J9L0
• Locus tag: K7B00_RS07150
• Protein ID: WP_009569577.1
• Coordinates: 1379024..1379530 (-)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: B7J9L1
• Locus tag: K7B00_RS07155
• Protein ID: WP_009569578.1
• Coordinates: 1379527..1379856 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7B00_RS07120 (K7B00_07120)	1374032..1374421	+	390	WP_009565421.1	holo-ACP synthase	-
K7B00_RS07125 (K7B00_07125)	1374425..1375747	+	1323	WP_012607056.1	UDP-glucose/GDP-mannose dehydrogenase family protein	-
K7B00_RS07130 (K7B00_07130)	1375728..1376699	+	972	WP_049759457.1	D-glycero-beta-D-manno-heptose-7-phosphate kinase	-
K7B00_RS07135 (K7B00_07135)	1376689..1377471	+	783	WP_012607057.1	ATP-binding protein	-
K7B00_RS07140 (K7B00_07140)	1377464..1378306	+	843	WP_012536503.1	Hsp33 family molecular chaperone HslO	-
K7B00_RS07150 (K7B00_07150)	1379024..1379530	-	507	WP_009569577.1	type II toxin-antitoxin system YhaV family toxin	Toxin
K7B00_RS07155 (K7B00_07155)	1379527..1379856	-	330	WP_009569578.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
K7B00_RS07160 (K7B00_07160)	1380143..1380286	+	144	WP_009569579.1	hypothetical protein	-
K7B00_RS07165 (K7B00_07165)	1380313..1380579	-	267	WP_012536504.1	type I restriction-modification system subunit M N-terminal domain-containing protein	-
K7B00_RS07170 (K7B00_07170)	1380924..1381418	-	495	WP_009565062.1	GNAT family N-acetyltransferase	-
K7B00_RS07175 (K7B00_07175)	1381415..1381687	-	273	WP_012536505.1	DUF1778 domain-containing protein	-
K7B00_RS07180 (K7B00_07180)	1381768..1381980	-	213	WP_009562915.1	hypothetical protein	-
K7B00_RS07185 (K7B00_07185)	1382148..1382360	-	213	WP_041645801.1	hypothetical protein	-
K7B00_RS07190 (K7B00_07190)	1382764..1383585	-	822	WP_012536506.1	UDP-2,3-diacylglucosamine diphosphatase	-
K7B00_RS07195 (K7B00_07195)	1383826..1384071	+	246	WP_009568482.1	DUF2934 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (16-152)

Antitoxin

• PrlF antitoxin (8-107)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 19373.17 Da
• Isoelectric Point: 9.9366

>T214443 WP_009569577.1 NZ_CP082238:c1379530-1379024 [Acidithiobacillus ferrooxidans]
MSSSKLAPLVIHGWTVFAHPLFLAHIETLAQQVEAFKKKDPVGYVKKNANKRLAAIIKLAFDAIPQDPTRPEYRQGNTLG
EDHKHWFRAKFFQQYRLFFRYHAPSKVIVLAWVNDEDTKRAYESSDDAYRVFRKMLESGRPPDDWNQLLAEACAEGQRLQ
RFAAGIAP

Nucleotide

• Length: 507 bp

>T214443 NZ_CP110074:2789111-2789251 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12107.54 Da
• Isoelectric Point: 4.3141

>AT214443 WP_009569578.1 NZ_CP082238:c1379856-1379527 [Acidithiobacillus ferrooxidans]
MAATLEVESTLTDRYQTTVPETVRRALRLGKRDKIHYTIRPGGEVVLTRAEDSEEDDPVLGQFLGFLARDIATHPERLQA
IDVSFVQRLQSLTGGIEVDLDAPLSADDE

Nucleotide

• Length: 330 bp

>AT214443 NZ_CP110074:c2789109-2788966 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAA

Structures

Toxin

• AlphaFold DB: B7J9L0

Antitoxin

• AlphaFold DB: B7J9L1