Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 1293564..1294161 Replicon chromosome
Accession NZ_CP082238
Organism Acidithiobacillus ferrooxidans strain BYM

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag K7B00_RS06650 Protein ID WP_054608787.1
Coordinates 1293564..1293842 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K7B00_RS06655 Protein ID WP_054608786.1
Coordinates 1293859..1294161 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7B00_RS06630 (K7B00_06630) 1288642..1290717 - 2076 WP_157293188.1 zincin-like metallopeptidase domain-containing protein -
K7B00_RS06635 (K7B00_06635) 1290698..1290877 - 180 WP_146235814.1 hypothetical protein -
K7B00_RS06640 (K7B00_06640) 1291017..1291952 - 936 WP_070807680.1 DNA adenine methylase -
K7B00_RS06645 (K7B00_06645) 1291956..1293407 - 1452 WP_054608788.1 toprim domain-containing protein -
K7B00_RS06650 (K7B00_06650) 1293564..1293842 + 279 WP_054608787.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K7B00_RS06655 (K7B00_06655) 1293859..1294161 + 303 WP_054608786.1 HigA family addiction module antitoxin Antitoxin
K7B00_RS06660 (K7B00_06660) 1294180..1296117 - 1938 WP_054608785.1 relaxase/mobilization nuclease domain-containing protein -
K7B00_RS06665 (K7B00_06665) 1296117..1296443 - 327 WP_054608784.1 hypothetical protein -
K7B00_RS06670 (K7B00_06670) 1296611..1296883 + 273 WP_054608783.1 HU family DNA-binding protein -
K7B00_RS06675 (K7B00_06675) 1296910..1297176 + 267 WP_054608782.1 superinfection immunity protein -
K7B00_RS06680 (K7B00_06680) 1297247..1297669 + 423 WP_054608781.1 hypothetical protein -
K7B00_RS06685 (K7B00_06685) 1297788..1298948 - 1161 WP_229129786.1 cellulose synthase complex periplasmic endoglucanase BcsZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-92)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10745.15 Da        Isoelectric Point: 7.8253

>T214441 WP_054608787.1 NZ_CP082238:1293564-1293842 [Acidithiobacillus ferrooxidans]
MIKSFRCKDTQSLFEGGAPRRFQGVRPQAERKLLMVDSAKDLKDLKSPPGNGLEKLSGDRKDQWSIRINDQWRICFDWRD
GDAYDVEIVDYH

Download         Length: 279 bp

>T214441 NZ_CP110074:c2783320-2782979 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11439.21 Da        Isoelectric Point: 10.8303

>AT214441 WP_054608786.1 NZ_CP082238:1293859-1294161 [Acidithiobacillus ferrooxidans]
MTRNRMRPIHPGEVLREEFLAPLGMTPHALAVALRVPSPRINDITRERRAITPDTALRLARYFGTTPEFWMELQSVYDLR
RAEVIAGESISREVTPRQAA

Download         Length: 303 bp

>AT214441 NZ_CP110074:c2783550-2783320 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References