Detailed information of TA system TA214437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 87342..87950
• Replicon: chromosome
• Accession: NZ_CP082238
• Organism: Acidithiobacillus ferrooxidans strain BYM

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2W1K1W3
• Locus tag: K7B00_RS00440
• Protein ID: WP_012535782.1
• Coordinates: 87342..87617 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: K7B00_RS00445
• Protein ID: WP_009566298.1
• Coordinates: 87633..87950 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7B00_RS00405 (K7B00_00405)	83120..84160	+	1041	WP_012535779.1	glycosyltransferase family 1 protein	-
K7B00_RS00410 (K7B00_00410)	84228..85154	+	927	WP_012535780.1	glycosyltransferase	-
K7B00_RS00415 (K7B00_00415)	85147..85515	+	369	WP_012535781.1	glycosyltransferase	-
K7B00_RS00425 (K7B00_00425)	86255..86476	+	222	WP_009566302.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K7B00_RS00430 (K7B00_00430)	86476..86889	+	414	WP_009566301.1	type II toxin-antitoxin system VapC family toxin	-
K7B00_RS00435 (K7B00_00435)	86886..87176	+	291	WP_009566300.1	hypothetical protein	-
K7B00_RS00440 (K7B00_00440)	87342..87617	+	276	WP_012535782.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K7B00_RS00445 (K7B00_00445)	87633..87950	+	318	WP_009566298.1	HigA family addiction module antitoxin	Antitoxin
K7B00_RS00450 (K7B00_00450)	87965..88144	+	180	WP_009566297.1	HNH endonuclease	-
K7B00_RS00455 (K7B00_00455)	88564..89727	+	1164	WP_009566294.1	Fic family protein	-
K7B00_RS00460 (K7B00_00460)	90024..90290	-	267	WP_196491178.1	hypothetical protein	-
K7B00_RS00465 (K7B00_00465)	90287..90529	-	243	WP_012535785.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
K7B00_RS00470 (K7B00_00470)	90613..90786	-	174	WP_009562770.1	PCP reductase family protein	-
K7B00_RS00475 (K7B00_00475)	90897..91325	-	429	WP_009562768.1	hypothetical protein	-
K7B00_RS00480 (K7B00_00480)	91328..91759	-	432	WP_009562767.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10433.84 Da
• Isoelectric Point: 7.9070

>T214437 WP_012535782.1 NZ_CP082238:87342-87617 [Acidithiobacillus ferrooxidans]
MIRSFVHKGLKQVFETGQSAKVGADLVTRCKRRLDAINAATSPEELNVPGFDFHALEGKPKRYTVHVNGPYCITFEWREG
DAWRIDLENYH

Nucleotide

• Length: 276 bp

>T214437 NZ_CP110074:571113-571229 [Enterococcus faecalis]
ATGTTGTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTAGTTTTGCAATACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11756.54 Da
• Isoelectric Point: 5.6631

>AT214437 WP_009566298.1 NZ_CP082238:87633-87950 [Acidithiobacillus ferrooxidans]
MDEMTVEQVDRIPTHPGEILREEVLPALDISVAEFARGLRVSRQTVHGILSERKGITPEMALRIGKYLGNGPGIWLRMQQ
AYDLTVAERRLAEELAAIHKPEQAA

Nucleotide

• Length: 318 bp

>AT214437 NZ_CP110074:c571345-571140 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTATTGCAAAACTAATCATCAGTCC

Structures

Toxin

• AlphaFold DB: A0A2W1K1W3