Detailed information of TA system TA214432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4130805..4131459
• Replicon: chromosome
• Accession: NZ_CP082236
• Organism: Pseudomonas sp. SC3(2021)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K5Y35_RS18470
• Protein ID: WP_123462954.1
• Coordinates: 4131109..4131459 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5Y35_RS18465
• Protein ID: WP_123462457.1
• Coordinates: 4130805..4131119 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5Y35_RS18445	4127171..4127371	+	201	WP_222832557.1	co-regulatory protein PtrA N-terminal domain-containing protein	-
K5Y35_RS18450	4127438..4127770	+	333	WP_222832559.1	DUF2790 domain-containing protein	-
K5Y35_RS18455	4127940..4129127	-	1188	WP_222832560.1	acyltransferase	-
K5Y35_RS18460	4129305..4130630	-	1326	WP_222832561.1	OprD family porin	-
K5Y35_RS18465	4130805..4131119	-	315	WP_123462457.1	transcriptional regulator	Antitoxin
K5Y35_RS18470	4131109..4131459	-	351	WP_123462954.1	toxin	Toxin
K5Y35_RS18475	4132038..4133501	-	1464	WP_007920173.1	NADH-quinone oxidoreductase subunit NuoN	-
K5Y35_RS18480	4133509..4135041	-	1533	WP_016986254.1	NADH-quinone oxidoreductase subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (46-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13906.75 Da
• Isoelectric Point: 8.3986

>T214432 WP_123462954.1 NZ_CP082236:c4131459-4131109 [Pseudomonas sp. SC3(2021)]
MDALFIELPVFQKHRDDYLDDDLFRSLQLELLKNPEAGDLIEDTGGLRKIRFSDQRRGKGKRSGLRVIYYWWSGFDQFWL
FTVYSKNEQDNLTPAQKKLFRKTLDKELNARTHYET

Nucleotide

• Length: 351 bp

>T214432 NZ_CP110072:c2711622-2711347 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11922.81 Da
• Isoelectric Point: 9.9896

>AT214432 WP_123462457.1 NZ_CP082236:c4131119-4130805 [Pseudomonas sp. SC3(2021)]
MKRDIFSELMDGLEALADERQGKITLRTHKVQLPKLMPITADEVVAIRQQLNLSRSVFAMYLRTNTRTLENWEQGRATPN
AQATTLIRLVERFPQTIEQLAALT

Nucleotide

• Length: 315 bp

>AT214432 NZ_CP110072:c2711881-2711615 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA