Detailed information of TA system TA214423

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): fst-ratA/Fst(toxin)
• Location: 51128..51369
• Replicon: plasmid pE006-101
• Accession: NZ_CP082232
• Organism: Enterococcus faecalis strain E006

Toxin (Protein)

• Gene name: fst
• Uniprot ID: -
• Locus tag: K4200_RS14080
• Protein ID: WP_002360667.1
• Coordinates: 51259..51369 (-)
• Length: 37 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	51128..51219 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4200_RS14060 (46853)	46853..47869	-	1017	WP_033593833.1	replication initiator protein A	-
K4200_RS14065 (48532)	48532..49383	+	852	WP_002362419.1	ParA family protein	-
K4200_RS14070 (49393)	49393..49935	+	543	WP_086321354.1	hypothetical protein	-
K4200_RS14075 (50720)	50720..51019	-	300	WP_010817802.1	hypothetical protein	-
- (51128)	51128..51219	+	92	NuclAT_0	-	Antitoxin
- (51128)	51128..51219	+	92	NuclAT_0	-	Antitoxin
- (51128)	51128..51219	+	92	NuclAT_0	-	Antitoxin
- (51128)	51128..51219	+	92	NuclAT_0	-	Antitoxin
K4200_RS14080 (51259)	51259..51369	-	111	WP_002360667.1	type I toxin-antitoxin system Fst family toxin	Toxin
K4200_RS14085 (51864)	51864..52181	+	318	WP_002394802.1	heavy metal-binding domain-containing protein	-
K4200_RS14090 (52217)	52217..52885	+	669	WP_002403283.1	CPBP family intramembrane metalloprotease	-
K4200_RS14095 (53002)	53002..53256	+	255	WP_002394800.1	hypothetical protein	-
K4200_RS14100 (53416)	53416..53649	-	234	WP_002394799.1	hypothetical protein	-
K4200_RS14105 (53651)	53651..53935	-	285	WP_002394798.1	hypothetical protein	-
K4200_RS14110 (53952)	53952..54572	-	621	WP_162500025.1	recombinase family protein	-
K4200_RS14115 (54776)	54776..55006	+	231	WP_002362431.1	hypothetical protein	-
K4200_RS14120 (55006)	55006..55347	+	342	WP_002362432.1	type II toxin-antitoxin system PemK/MazF family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / dfrG / lnu(G) / erm(A) / optrA / ant(6)-Ia	prgB/asc10	1..101692	101692

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (3-35)

Sequences

Toxin

Protein

• Length: 37 a.a.
• Molecular weight: 4117.92 Da
• Isoelectric Point: 4.1672

>T214423 WP_002360667.1 NZ_CP082232:c51369-51259 [Enterococcus faecalis]
VLFVKDLMSLVIAPIFVGLVLEMISRVLDEEDDNRK

Nucleotide

• Length: 111 bp

>T214423 NZ_CP110071:c2404804-2404688 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Nucleotide

• Length: 92 bp

>AT214423 NZ_CP082232:51128-51219 [Enterococcus faecalis]
TAAAAATATGTTATACTAAAGGTGCGAAACGACATTAAATCGTACAAATAACACAAAAAGCAATCCTACGGCGAATAGGA
TTGCTTTTTTTT

Structures

Antitoxin