Detailed information of TA system TA214396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 12431..13089
• Replicon: plasmid p2018TJAB1
• Accession: NZ_CP082221
• Organism: Acinetobacter baumannii strain 2018TJAB1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H1I74_RS19025
• Protein ID: WP_000312250.1
• Coordinates: 12730..13089 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H1I74_RS19020
• Protein ID: WP_001096429.1
• Coordinates: 12431..12730 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1I74_RS18985	7598..8227	+	630	WP_002081923.1	hypothetical protein	-
H1I74_RS18990	8267..8776	+	510	WP_001043199.1	hypothetical protein	-
H1I74_RS18995	8858..9370	+	513	WP_000840065.1	hypothetical protein	-
H1I74_RS19000	9426..9755	+	330	WP_000459117.1	hypothetical protein	-
H1I74_RS19005	9792..10520	+	729	WP_001022819.1	hypothetical protein	-
H1I74_RS19010	10949..11308	+	360	WP_223234021.1	hypothetical protein	-
H1I74_RS19015	11358..11894	+	537	WP_060853649.1	hypothetical protein	-
H1I74_RS19020	12431..12730	-	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
H1I74_RS19025	12730..13089	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H1I74_RS19030	13290..13856	+	567	WP_031948745.1	hypothetical protein	-
H1I74_RS19035	13905..14087	+	183	WP_000373384.1	hypothetical protein	-
H1I74_RS19040	14154..14852	+	699	WP_000873188.1	hypothetical protein	-
H1I74_RS19045	14991..15374	+	384	WP_000654347.1	hypothetical protein	-
H1I74_RS19050	15441..16199	+	759	WP_060853650.1	hypothetical protein	-
H1I74_RS19055	16251..16586	+	336	WP_000654055.1	hypothetical protein	-
H1I74_RS19060	17451..17765	+	315	WP_000708713.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-23	-	1..78022	78022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T214396 WP_000312250.1 NZ_CP082221:c13089-12730 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T214396 NZ_CP110063:2816301-2816444 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGAAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT214396 WP_001096429.1 NZ_CP082221:c12730-12431 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT214396 NZ_CP110063:c2816204-2816003 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA