Detailed information of TA system TA214389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 86963..87552
• Replicon: chromosome
• Accession: NZ_CP082218
• Organism: Xanthomonas cucurbitae strain IL_234

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: K6979_RS00355
• Protein ID: WP_104603499.1
• Coordinates: 86963..87244 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6979_RS00360
• Protein ID: WP_104603498.1
• Coordinates: 87262..87552 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6979_RS00350 (K6979_00355)	84679..86865	+	2187	WP_104603500.1	exodeoxyribonuclease V subunit alpha	-
K6979_RS00355 (K6979_00360)	86963..87244	+	282	WP_104603499.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6979_RS00360 (K6979_00365)	87262..87552	+	291	WP_104603498.1	HigA family addiction module antitoxin	Antitoxin
K6979_RS00365 (K6979_00370)	87561..87713	-	153	WP_274393848.1	hypothetical protein	-
K6979_RS00370	87824..88005	-	182	Protein_73	hypothetical protein	-
K6979_RS00375 (K6979_00375)	88521..89054	+	534	WP_211292995.1	DUF3800 domain-containing protein	-
K6979_RS00380 (K6979_00380)	89259..89591	-	333	WP_104603496.1	hypothetical protein	-
K6979_RS00385 (K6979_00385)	90191..90520	+	330	WP_104603495.1	DUF1778 domain-containing protein	-
K6979_RS00390 (K6979_00390)	90468..91013	+	546	WP_104603494.1	GNAT family N-acetyltransferase	-
K6979_RS00395 (K6979_00395)	91080..91283	+	204	Protein_78	IS4 family transposase	-
K6979_RS00400 (K6979_00400)	91334..92134	-	801	WP_274393849.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11012.71 Da
• Isoelectric Point: 10.2909

>T214389 WP_104603499.1 NZ_CP082218:86963-87244 [Xanthomonas cucurbitae]
MIRSFIDKEAERIWLGERSRRLPADIQPVARRKLRMLNAAAHLDDLRIPPANRLEALKGARRGQYSIRINDQWRICFRWM
EGDAAEVEIVDYH

Nucleotide

• Length: 282 bp

>T214389 NZ_CP110063:563057-563173 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10464.13 Da
• Isoelectric Point: 7.2043

>AT214389 WP_104603498.1 NZ_CP082218:87262-87552 [Xanthomonas cucurbitae]
MTVLPNIHPGEILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTTERFWLGLQADYELEQA
HRALGDLPSRIKRLAA

Nucleotide

• Length: 291 bp

>AT214389 NZ_CP110063:c563289-563084 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC