Detailed information of TA system TA214380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1901440..1901955
• Replicon: chromosome
• Accession: NZ_CP082215
• Organism: Xanthomonas cucurbitae strain MI_359

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: K6982_RS08145
• Protein ID: WP_167526871.1
• Coordinates: 1901440..1901646 (+)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6982_RS08150
• Protein ID: WP_159406979.1
• Coordinates: 1901656..1901955 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6982_RS08115 (K6982_08085)	1896542..1896907	-	366	WP_002806565.1	response regulator	-
K6982_RS08120 (K6982_08090)	1896904..1897212	-	309	WP_104604422.1	STAS domain-containing protein	-
K6982_RS08125 (K6982_08095)	1897327..1898541	-	1215	WP_159406977.1	chemotaxis protein CheW	-
K6982_RS08130 (K6982_08100)	1898538..1899320	-	783	WP_159406978.1	ParA family protein	-
K6982_RS08135 (K6982_08105)	1899322..1900296	-	975	WP_104604428.1	flagellar motor protein MotD	-
K6982_RS08140 (K6982_08110)	1900304..1901044	-	741	WP_104604430.1	flagellar motor protein	-
K6982_RS08145 (K6982_08115)	1901440..1901646	+	207	WP_167526871.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6982_RS08150 (K6982_08120)	1901656..1901955	+	300	WP_159406979.1	HigA family addiction module antitoxin	Antitoxin
K6982_RS08155 (K6982_08125)	1902107..1903705	+	1599	WP_159406980.1	S41 family peptidase	-
K6982_RS08160 (K6982_08130)	1903986..1904771	-	786	WP_126942758.1	hypothetical protein	-
K6982_RS08165 (K6982_08135)	1904755..1905294	-	540	WP_223214429.1	HNH endonuclease signature motif containing protein	-
K6982_RS08170 (K6982_08140)	1905390..1906406	-	1017	WP_159406982.1	IS30 family transposase	-
K6982_RS08175 (K6982_08145)	1906382..1906765	-	384	WP_159406983.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	cheY	1712118..1956071	243953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-68)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (7-83)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7668.74 Da
• Isoelectric Point: 7.2776

>T214380 WP_167526871.1 NZ_CP082215:1901440-1901646 [Xanthomonas cucurbitae]
MQAAAERKLQLLDSAQTLEFLRSPPANRLELLAGTRVGQHSIRINDQWRVCFVWTGAGPGHVEIVDYH

Nucleotide

• Length: 207 bp

>T214380 NZ_CP082215:1901440-1901646 [Xanthomonas cucurbitae]
ATGCAGGCCGCAGCCGAGCGGAAGCTGCAGTTGCTGGATTCGGCGCAGACGCTGGAGTTCTTGCGCAGTCCGCCAGCCAA
TCGGCTTGAGCTTCTTGCAGGTACGCGTGTAGGTCAGCACAGCATCCGCATCAACGACCAATGGCGTGTGTGCTTTGTCT
GGACCGGTGCCGGCCCTGGGCACGTAGAGATTGTTGACTATCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10831.47 Da
• Isoelectric Point: 10.7850

>AT214380 WP_159406979.1 NZ_CP082215:1901656-1901955 [Xanthomonas cucurbitae]
MRPTNKLRAIHPGEILREDFMLPLQLTINGLARALDVPATRVHSIVHGARAITADTAARLARHFGGDAASWLALQATYDL
KTLPNRSEIETNVQPRVAA

Nucleotide

• Length: 300 bp

>AT214380 NZ_CP082215:1901656-1901955 [Xanthomonas cucurbitae]
ATGCGACCCACCAACAAGCTGCGCGCGATCCATCCCGGCGAGATCCTGCGCGAGGACTTCATGCTGCCATTGCAGCTGAC
CATCAACGGCCTGGCGCGTGCGTTGGATGTGCCTGCCACGCGCGTCCACAGCATCGTGCACGGCGCGCGCGCGATCACTG
CCGATACCGCTGCGCGCCTTGCGCGTCACTTCGGTGGCGACGCGGCATCCTGGCTTGCGCTGCAGGCCACTTACGACCTG
AAGACCTTGCCCAACCGTAGTGAGATCGAGACCAACGTGCAGCCGCGTGTAGCGGCGTAG