Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 390389..391035 Replicon chromosome
Accession NZ_CP082212
Organism Klebsiella pneumoniae strain BJ20

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag K6039_RS01885 Protein ID WP_002920560.1
Coordinates 390389..390736 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag K6039_RS01890 Protein ID WP_002920557.1
Coordinates 390736..391035 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6039_RS01875 (K6039_01865) 386315..387748 + 1434 WP_002920564.1 glycogen synthase GlgA -
K6039_RS01880 (K6039_01870) 387766..390213 + 2448 WP_002920561.1 glycogen phosphorylase -
K6039_RS01885 (K6039_01875) 390389..390736 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K6039_RS01890 (K6039_01880) 390736..391035 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
K6039_RS01895 (K6039_01885) 391098..392606 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
K6039_RS01900 (K6039_01890) 392811..393140 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
K6039_RS01905 (K6039_01895) 393191..394021 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
K6039_RS01910 (K6039_01900) 394071..394829 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T214352 WP_002920560.1 NZ_CP082212:390389-390736 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T214352 NZ_CP110050:c2809149-2808808 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT214352 WP_002920557.1 NZ_CP082212:390736-391035 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT214352 NZ_CP110050:c2809379-2809149 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References