Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3910792..3911389 Replicon chromosome
Accession NZ_CP082211
Organism Klebsiella pneumoniae strain A1718

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag K6036_RS18920 Protein ID WP_004142563.1
Coordinates 3911072..3911389 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag K6036_RS18915 Protein ID WP_004142561.1
Coordinates 3910792..3911079 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6036_RS18885 (3906872) 3906872..3907120 + 249 WP_020325243.1 DUF1158 domain-containing protein -
K6036_RS18890 (3907138) 3907138..3907479 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
K6036_RS18895 (3907510) 3907510..3908625 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
K6036_RS18900 (3908805) 3908805..3909386 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
K6036_RS18905 (3909386) 3909386..3909754 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
K6036_RS18910 (3909874) 3909874..3910527 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
K6036_RS18915 (3910792) 3910792..3911079 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
K6036_RS18920 (3911072) 3911072..3911389 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K6036_RS18925 (3911574) 3911574..3912617 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
K6036_RS18930 (3913286) 3913286..3914152 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
K6036_RS18935 (3914261) 3914261..3915688 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T214344 WP_004142563.1 NZ_CP082211:c3911389-3911072 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T214344 NZ_CP110048:21591-21866 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCATGCACTAACAGGAAATTATA
TAGGTACACGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT214344 WP_004142561.1 NZ_CP082211:c3911079-3910792 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT214344 NZ_CP110048:21332-21598 [Enterococcus faecalis]
TTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACTATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAACGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References