Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 323790..324376 Replicon chromosome
Accession NZ_CP082211
Organism Klebsiella pneumoniae strain A1718

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag K6036_RS01510 Protein ID WP_002920800.1
Coordinates 324008..324376 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag K6036_RS01505 Protein ID WP_004174006.1
Coordinates 323790..324011 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6036_RS01485 (319947) 319947..320873 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
K6036_RS01490 (320870) 320870..322147 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
K6036_RS01495 (322144) 322144..322911 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
K6036_RS01500 (322913) 322913..323626 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
K6036_RS01505 (323790) 323790..324011 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K6036_RS01510 (324008) 324008..324376 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K6036_RS01515 (324649) 324649..325965 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
K6036_RS01520 (326072) 326072..326959 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
K6036_RS01525 (326956) 326956..327801 + 846 WP_004181473.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
K6036_RS01530 (327803) 327803..328873 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 320870..329610 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T214332 WP_002920800.1 NZ_CP082211:324008-324376 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T214332 NZ_CP110043:27945-28046 [Enterococcus faecalis]
GTGAAAGATTTAATGTCGTTGGTTATCGCACCGATCTTTGTAGGATTGGTTCTGGAAATGATTTCTCGTGTGTTGGACGA
GGAAGACGATAGCCGAAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT214332 WP_004174006.1 NZ_CP082211:323790-324011 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT214332 NZ_CP110043:c28150-28086 [Enterococcus faecalis]
AACGACATTAAATCGTACAAATAACACAAAAAGCAATCCTACGGCGAATAGGATTGCTTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References