Detailed information of TA system TA214270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4034757..4035347
• Replicon: chromosome
• Accession: NZ_CP082178
• Organism: Xanthomonas fragariae strain SHQP01

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2N7V9W3
• Locus tag: K4A87_RS19460
• Protein ID: WP_016902142.1
• Coordinates: 4035066..4035347 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: K4A87_RS19455
• Protein ID: WP_002803896.1
• Coordinates: 4034757..4035047 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4A87_RS19435 (K4A87_19295)	4031948..4032853	-	906	WP_208588208.1	hypothetical protein	-
K4A87_RS19440 (K4A87_19300)	4032916..4033443	-	528	WP_208588209.1	hypothetical protein	-
K4A87_RS19445 (K4A87_19305)	4033883..4034215	+	333	WP_208588210.1	hypothetical protein	-
K4A87_RS19450	4034376..4034566	+	191	Protein_3601	hypothetical protein	-
K4A87_RS19455 (K4A87_19310)	4034757..4035047	-	291	WP_002803896.1	HigA family addiction module antitoxin	Antitoxin
K4A87_RS19460 (K4A87_19315)	4035066..4035347	-	282	WP_016902142.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K4A87_RS19465 (K4A87_19320)	4035809..4037944	-	2136	WP_208588211.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-94)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11064.75 Da
• Isoelectric Point: 9.7972

>T214270 WP_016902142.1 NZ_CP082178:c4035347-4035066 [Xanthomonas fragariae]
MIRSFVDKEAEKIWMGERSRRLPADIQSVARRKLRMLNAAAHLDDLRIPPANRLEALKGERRGQYSIRINDQWRICFRWM
EGDVAEVEIVDYH

Nucleotide

• Length: 282 bp

>T214270 NZ_CP110018:c220343-220065 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10528.25 Da
• Isoelectric Point: 7.1667

>AT214270 WP_002803896.1 NZ_CP082178:c4035047-4034757 [Xanthomonas fragariae]
MTVLPNIHPGEILLEEFLEPMGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALCTTERFWLGLQADYELEQA
HRALGDLPSRIKRLAA

Nucleotide

• Length: 291 bp

>AT214270 NZ_CP110018:c220606-220346 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A2N7V9W3