Detailed information of TA system TA214252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 7720966..7721599
• Replicon: chromosome
• Accession: NZ_CP082160
• Organism: Rhodococcus opacus strain DSM 44186

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A3A9TJ70
• Locus tag: FXW36_RS39290
• Protein ID: WP_054248704.1
• Coordinates: 7721318..7721599 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FXW36_RS39285
• Protein ID: WP_007297375.1
• Coordinates: 7720966..7721259 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXW36_RS39265 (FXW36_39260)	7716345..7717160	+	816	WP_061700505.1	AAA family ATPase	-
FXW36_RS39270 (FXW36_39265)	7717438..7718985	-	1548	WP_054248794.1	sulfatase-like hydrolase/transferase	-
FXW36_RS39275 (FXW36_39270)	7719294..7720031	-	738	WP_120659161.1	hypothetical protein	-
FXW36_RS39280 (FXW36_39275)	7720142..7720744	-	603	WP_009480668.1	recombinase family protein	-
FXW36_RS39285 (FXW36_39280)	7720966..7721259	-	294	WP_007297375.1	HigA family addiction module antitoxin	Antitoxin
FXW36_RS39290 (FXW36_39285)	7721318..7721599	-	282	WP_054248704.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FXW36_RS41985	7721842..7721973	-	132	WP_009480666.1	hypothetical protein	-
FXW36_RS39295 (FXW36_39290)	7722262..7722750	+	489	WP_043782979.1	hypothetical protein	-
FXW36_RS39300 (FXW36_39295)	7722952..7723728	-	777	WP_222820791.1	pentapeptide repeat-containing protein	-
FXW36_RS39305 (FXW36_39300)	7724054..7726408	+	2355	WP_120659159.1	ATP-binding cassette domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-81)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11006.51 Da
• Isoelectric Point: 8.9336

>T214252 WP_054248704.1 NZ_CP082160:c7721599-7721318 [Rhodococcus opacus]
VIESFADKETERVFHRQFSKKLPQDIQRIARRRLLLIEAATDVNDLRVPPGNRLEKLSGDRLGQYSVRINDQYRICFIWR
ATAAYDVEITDYH

Nucleotide

• Length: 282 bp

>T214252 NZ_CP110017:1597896-1598072 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10745.39 Da
• Isoelectric Point: 7.0123

>AT214252 WP_007297375.1 NZ_CP082160:c7721259-7720966 [Rhodococcus opacus]
MTTDLSPIHPGEILREDFLEPFGITQNRLATIIGVPPRRINEIVLGKRSITADTALRLAKAFGNSAQFWLNIQDRYELDL
AADKIGDALAKITRLSA

Nucleotide

• Length: 294 bp

>AT214252 NZ_CP110017:1598118-1598534 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A3A9TJ70