Detailed information of TA system TA021423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 252005..252699
• Replicon: chromosome
• Accession: NC_007779
• Organism: Escherichia coli str. K-12 substr. W3110

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: Y75_RS01190
• Protein ID: WP_001263489.1
• Coordinates: 252301..252699 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: Y75_RS01185
• Protein ID: WP_000554758.1
• Coordinates: 252005..252298 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y75_RS01165 (Y75_p0219)	247637..248134	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
Y75_RS01170 (Y75_p0220)	248358..250070	-	1713	Protein_224	flagellar biosynthesis protein FlhA	-
Y75_RS01175 (Y75_p0221)	250042..250827	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
Y75_RS01180 (Y75_p0222)	250898..251953	+	1056	WP_001226164.1	DNA polymerase IV	-
Y75_RS01185 (Y75_p0223)	252005..252298	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
Y75_RS01190 (Y75_p0224)	252301..252699	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
Y75_RS01195 (Y75_p0225)	252709..253161	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
Y75_RS01200 (Y75_p0226)	253479..253685	+	207	Protein_230	RtcB family protein	-
Y75_RS01205 (Y75_p0227)	253681..254202	+	522	Protein_231	peptide chain release factor H	-
Y75_RS01210 (Y75_p0228)	254259..255716	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
Y75_RS01215 (Y75_p0229)	255977..256435	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T21423 WP_001263489.1 NC_007779:252301-252699 [Escherichia coli str. K-12 substr. W3110]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T21423 NC_007779:252301-252699 [Escherichia coli str. K-12 substr. W3110]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT21423 WP_000554758.1 NC_007779:252005-252298 [Escherichia coli str. K-12 substr. W3110]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT21423 NC_007779:252005-252298 [Escherichia coli str. K-12 substr. W3110]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3