Detailed information of TA system TA214226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4795068..4795670
• Replicon: chromosome
• Accession: NZ_CP082147
• Organism: Enterobacter cloacae complex sp. strain ECL42

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K6966_RS23165
• Protein ID: WP_058690340.1
• Coordinates: 4795359..4795670 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B2XUJ3
• Locus tag: K6966_RS23160
• Protein ID: WP_032635182.1
• Coordinates: 4795068..4795358 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6966_RS23145 (K6966_23145)	4792566..4793468	+	903	WP_014833793.1	formate dehydrogenase subunit beta	-
K6966_RS23150 (K6966_23150)	4793465..4794100	+	636	WP_008501832.1	formate dehydrogenase cytochrome b556 subunit	-
K6966_RS23155 (K6966_23155)	4794097..4795026	+	930	WP_014885738.1	formate dehydrogenase accessory protein FdhE	-
K6966_RS23160 (K6966_23160)	4795068..4795358	-	291	WP_032635182.1	NadS family protein	Antitoxin
K6966_RS23165 (K6966_23165)	4795359..4795670	-	312	WP_058690340.1	hypothetical protein	Toxin
K6966_RS23170 (K6966_23170)	4795899..4796819	+	921	WP_058690339.1	alpha/beta hydrolase	-
K6966_RS23175 (K6966_23175)	4796828..4797769	-	942	WP_023336483.1	fatty acid biosynthesis protein FabY	-
K6966_RS23180 (K6966_23180)	4797814..4798251	-	438	WP_014885747.1	D-aminoacyl-tRNA deacylase	-
K6966_RS23185 (K6966_23185)	4798248..4799129	-	882	WP_047343713.1	virulence factor BrkB family protein	-
K6966_RS23190 (K6966_23190)	4799123..4799722	-	600	WP_200923749.1	glucose-1-phosphatase	-
K6966_RS23195 (K6966_23195)	4799842..4800642	-	801	WP_047343714.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12264.32 Da
• Isoelectric Point: 10.1230

>T214226 WP_058690340.1 NZ_CP082147:c4795670-4795359 [Enterobacter cloacae complex sp.]
MLFIETDIFTEDVKTLLDDDEYHKLQVFLVTQPNYGDLIQNTGGLRKIRWFSGSKGKRGGVRVIYFHRTREFEIRLLLIY
RKGIKDDLSAKEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T214226 NZ_CP110016:1597897-1598073 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10703.22 Da
• Isoelectric Point: 10.5888

>AT214226 WP_032635182.1 NZ_CP082147:c4795358-4795068 [Enterobacter cloacae complex sp.]
MDKALFERLTQSIAQMNEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALSQ

Nucleotide

• Length: 291 bp

>AT214226 NZ_CP110016:1598119-1598535 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A8B2XUJ3