Detailed information of TA system TA021421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4724702..4725297
• Replicon: chromosome
• Accession: NC_007778
• Organism: Rhodopseudomonas palustris HaA2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q2ISH1
• Locus tag: RPB_RS20885
• Protein ID: WP_011443023.1
• Coordinates: 4724702..4724980 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RPB_RS20890
• Protein ID: WP_011443024.1
• Coordinates: 4724992..4725297 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RPB_RS20880	4721654..4724644	+	2991	WP_080507813.1	excinuclease ABC subunit UvrB	-
RPB_RS20885	4724702..4724980	+	279	WP_011443023.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RPB_RS20890	4724992..4725297	+	306	WP_011443024.1	HigA family addiction module antidote protein	Antitoxin
RPB_RS20895	4725341..4725586	+	246	WP_011443025.1	transcriptional regulator	-
RPB_RS20900	4725601..4725921	-	321	WP_041798390.1	(2Fe-2S) ferredoxin domain-containing protein	-
RPB_RS20905	4726209..4727462	-	1254	WP_011443027.1	acetyl-CoA C-acetyltransferase	-
RPB_RS20910	4727623..4728018	+	396	WP_011443028.1	hypothetical protein	-
RPB_RS20915	4728454..4728885	+	432	WP_011443029.1	nuclear transport factor 2 family protein	-
RPB_RS20920	4729066..4729509	+	444	WP_011443030.1	hypothetical protein	-
RPB_RS24965	4729650..4729799	-	150	WP_011443031.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10944.44 Da
• Isoelectric Point: 9.0805

>T21421 WP_011443023.1 NC_007778:4724702-4724980 [Rhodopseudomonas palustris HaA2]
MIRSFRNQATARLFADEDVPRFRAIERQARRKLLLLDGAAKLDDLRQPPGNRLEALKGDRRGQYSIRINDQWRICFEWRD
DGAEMVEILDYH

Nucleotide

• Length: 279 bp

>T21421 NC_007778:4724702-4724980 [Rhodopseudomonas palustris HaA2]
ATGATCCGCTCGTTCCGGAACCAGGCGACCGCGCGGTTGTTTGCCGACGAGGACGTGCCGCGCTTTCGTGCCATCGAGCG
GCAGGCCCGGCGCAAATTGCTCCTGCTGGATGGTGCCGCGAAGCTGGATGATCTGCGCCAGCCTCCCGGCAACAGACTCG
AAGCCCTGAAAGGGGATCGCCGCGGTCAATACTCGATCCGCATCAACGATCAATGGCGGATCTGCTTCGAATGGCGCGAC
GACGGCGCAGAGATGGTCGAGATTTTGGACTATCACTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11301.07 Da
• Isoelectric Point: 9.8872

>AT21421 WP_011443024.1 NC_007778:4724992-4725297 [Rhodopseudomonas palustris HaA2]
MATPKQLPPIPPGEILIEEFMRPNGISQNRLARDIDINPARVNDIVHGRSAITASVALRLAKYFGTTPELWMNLQASYDL
RRARAADWPAIEPRVRVLAAE

Nucleotide

• Length: 306 bp

>AT21421 NC_007778:4724992-4725297 [Rhodopseudomonas palustris HaA2]
ATGGCCACACCAAAGCAGCTCCCCCCGATCCCTCCCGGCGAGATTCTGATCGAGGAATTCATGCGTCCGAACGGCATCAG
CCAAAATAGGCTGGCACGCGATATCGACATCAACCCGGCCCGGGTCAACGACATCGTGCACGGCCGCTCTGCGATCACCG
CTTCCGTCGCCCTGCGGCTGGCGAAGTATTTCGGCACGACGCCAGAACTGTGGATGAACCTGCAGGCCTCCTACGATCTG
CGCCGCGCCCGCGCCGCAGACTGGCCCGCGATCGAGCCGCGCGTCCGCGTGCTCGCCGCGGAGTAG

Structures

Toxin

• AlphaFold DB: Q2ISH1