Detailed information of TA system TA214209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 61443..62089
• Replicon: chromosome
• Accession: NZ_CP082147
• Organism: Enterobacter cloacae complex sp. strain ECL42

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K6966_RS00330
• Protein ID: WP_058690226.1
• Coordinates: 61739..62089 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6966_RS00325
• Protein ID: WP_032635284.1
• Coordinates: 61443..61742 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6966_RS00295 (K6966_00295)	56857..57816	-	960	WP_023336511.1	DUF523 and DUF1722 domain-containing protein	-
K6966_RS00300 (K6966_00300)	57919..58329	-	411	WP_014881954.1	hydroxyisourate hydrolase	-
K6966_RS00305 (K6966_00305)	58429..59154	-	726	WP_014881955.1	MerR family transcriptional regulator	-
K6966_RS00310 (K6966_00310)	59272..59796	+	525	WP_014881956.1	lipocalin family protein	-
K6966_RS00315 (K6966_00315)	59875..60873	+	999	WP_222834734.1	class I SAM-dependent methyltransferase	-
K6966_RS00320 (K6966_00320)	60976..61413	+	438	WP_058690221.1	acetyltransferase	-
K6966_RS00325 (K6966_00325)	61443..61742	-	300	WP_032635284.1	XRE family transcriptional regulator	Antitoxin
K6966_RS00330 (K6966_00330)	61739..62089	-	351	WP_058690226.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6966_RS26950	62353..62533	-	181	Protein_65	hydroxyacid dehydrogenase	-
K6966_RS00335 (K6966_00335)	62620..62961	-	342	WP_058690222.1	SymE family type I addiction module toxin	-
K6966_RS00340 (K6966_00340)	63090..63251	+	162	WP_072205328.1	YlcI/YnfO family protein	-
K6966_RS00345 (K6966_00345)	63497..64012	+	516	WP_222834735.1	hypothetical protein	-
K6966_RS00350 (K6966_00350)	64032..64478	-	447	WP_222834736.1	hypothetical protein	-
K6966_RS00355 (K6966_00355)	64460..65440	-	981	WP_200923523.1	IS5-like element ISKpn26 family transposase	-
K6966_RS00360 (K6966_00360)	65439..65909	+	471	WP_222834737.1	hypothetical protein	-
K6966_RS00365 (K6966_00365)	65975..66280	-	306	WP_078309521.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	64460..65440	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13481.52 Da
• Isoelectric Point: 6.9819

>T214209 WP_058690226.1 NZ_CP082147:c62089-61739 [Enterobacter cloacae complex sp.]
MWTVLFGKVFELWLLEQEEGLQEKVLADMLNLQNFGPRLPRPYADTVKGSQYNNMKELRIQYAGRPIRALFAFDPERQAI
VLCAGNKSNDKAFYEKLIRIADDEFTRHLTSLETAK

Nucleotide

• Length: 351 bp

>T214209 NZ_CP110015:c3002994-3002659 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11160.04 Da
• Isoelectric Point: 6.9842

>AT214209 WP_032635284.1 NZ_CP082147:c61742-61443 [Enterobacter cloacae complex sp.]
VKTLNELIASRSPESQQRIKEMAEEMILETGLQLLREELRLSQKNVAELMGVSQPAVTQIEQRGNELKIATLKRYIEAMG
GKLCIDVELPTGKRVAFHI

Nucleotide

• Length: 300 bp

>AT214209 NZ_CP110015:c3003242-3002994 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA