Detailed information of TA system TA214208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31715..32445
• Replicon: chromosome
• Accession: NZ_CP082147
• Organism: Enterobacter cloacae complex sp. strain ECL42

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2W0GI63
• Locus tag: K6966_RS00155
• Protein ID: WP_023331686.1
• Coordinates: 31715..32029 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K6966_RS00160
• Protein ID: WP_127341140.1
• Coordinates: 32026..32445 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6966_RS00130 (K6966_00130)	27380..28468	+	1089	WP_032635252.1	cellulase family glycosylhydrolase	-
K6966_RS00135 (K6966_00135)	28371..29555	-	1185	WP_222834732.1	multidrug efflux MFS transporter EmrD	-
K6966_RS00140 (K6966_00140)	29731..30564	-	834	WP_045134488.1	EamA family transporter	-
K6966_RS00145 (K6966_00145)	30627..31073	-	447	WP_014881927.1	GNAT family N-acetyltransferase	-
K6966_RS00155 (K6966_00155)	31715..32029	+	315	WP_023331686.1	type II toxin-antitoxin system HigB family toxin	Toxin
K6966_RS00160 (K6966_00160)	32026..32445	+	420	WP_127341140.1	helix-turn-helix domain-containing protein	Antitoxin
K6966_RS00165 (K6966_00165)	32595..32693	+	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
K6966_RS00170 (K6966_00170)	32800..34488	+	1689	WP_047028139.1	acetolactate synthase large subunit	-
K6966_RS00175 (K6966_00175)	34492..34779	+	288	WP_010426528.1	acetolactate synthase small subunit	-
K6966_RS00180 (K6966_00180)	34862..35455	+	594	WP_014881931.1	transcriptional regulator UhpA	-
K6966_RS00185 (K6966_00185)	35452..36957	+	1506	WP_014881932.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.59 Da
• Isoelectric Point: 10.2825

>T214208 WP_023331686.1 NZ_CP082147:31715-32029 [Enterobacter cloacae complex sp.]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCTTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T214208 NZ_CP110015:2868580-2868909 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15533.13 Da
• Isoelectric Point: 5.6761

>AT214208 WP_127341140.1 NZ_CP082147:32026-32445 [Enterobacter cloacae complex sp.]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMASRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT214208 NZ_CP110015:2868242-2868559 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A2W0GI63