Detailed information of TA system TA214201

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 94398..94819
• Replicon: plasmid pIncFIA
• Accession: NZ_CP082130
• Organism: Escherichia coli strain NDM_11.16372

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K5P77_RS24050
• Protein ID: WP_001375150.1
• Coordinates: 94703..94819 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	94398..94596 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P77_RS24015 (90094)	90094..91488	+	1395	WP_001037799.1	carbohydrate porin	-
K5P77_RS24020 (91734)	91734..91838	-	105	Protein_98	thiosulfate reductase cytochrome B subunit	-
K5P77_RS24025 (91895)	91895..92588	+	694	Protein_99	IS1 family transposase	-
K5P77_RS24030 (92715)	92715..93227	+	513	WP_223350513.1	potassium channel family protein	-
K5P77_RS24035 (93312)	93312..93563	+	252	Protein_101	iron-containing alcohol dehydrogenase	-
K5P77_RS24040 (93852)	93852..94169	-	318	Protein_102	IS110 family transposase	-
K5P77_RS24045 (94324)	94324..94429	+	106	Protein_103	plasmid SOS inhibition protein A	-
- (94398)	94398..94596	+	199	NuclAT_0	-	Antitoxin
- (94398)	94398..94596	+	199	NuclAT_0	-	Antitoxin
- (94398)	94398..94596	+	199	NuclAT_0	-	Antitoxin
- (94398)	94398..94596	+	199	NuclAT_0	-	Antitoxin
K5P77_RS24580 (94603)	94603..94752	+	150	Protein_104	DUF5431 family protein	-
K5P77_RS24050 (94703)	94703..94819	+	117	WP_001375150.1	type I toxin-antitoxin system Hok family toxin	Toxin
K5P77_RS24585 (95120)	95120..95417	-	298	Protein_106	hypothetical protein	-
K5P77_RS24055 (95487)	95487..95693	+	207	WP_000275854.1	hypothetical protein	-
K5P77_RS24060 (95718)	95718..96005	+	288	WP_000107242.1	hypothetical protein	-
K5P77_RS24065 (96124)	96124..96945	+	822	WP_001234465.1	DUF932 domain-containing protein	-
K5P77_RS24070 (97241)	97241..97813	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
K5P77_RS24075 (98183)	98183..98566	+	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K5P77_RS24080 (98757)	98757..99404	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
K5P77_RS24085 (99540)	99540..99755	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A)	-	1..136961	136961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4481.27 Da
• Isoelectric Point: 8.4890

>T214201 WP_001375150.1 NZ_CP082130:94703-94819 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T214201 NZ_CP110015:c1679816-1678494 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT214201 NZ_CP082130:94398-94596 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin