Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3834188..3834729 Replicon chromosome
Accession NZ_CP082129
Organism Escherichia coli strain NDM_11.16372

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag K5P77_RS18550 Protein ID WP_000615983.1
Coordinates 3834451..3834729 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag K5P77_RS18545 Protein ID WP_000729703.1
Coordinates 3834188..3834448 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P77_RS18525 (3829863) 3829863..3830648 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
K5P77_RS18530 (3830620) 3830620..3832332 + 1713 Protein_3649 flagellar biosynthesis protein FlhA -
K5P77_RS18535 (3832556) 3832556..3833053 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
K5P77_RS18540 (3833229) 3833229..3833978 - 750 WP_000056849.1 C40 family peptidase -
K5P77_RS18545 (3834188) 3834188..3834448 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K5P77_RS18550 (3834451) 3834451..3834729 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K5P77_RS18555 (3834885) 3834885..3835625 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K5P77_RS18560 (3835596) 3835596..3836363 - 768 WP_000333380.1 class II glutamine amidotransferase -
K5P77_RS18565 (3836569) 3836569..3837147 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T214195 WP_000615983.1 NZ_CP082129:3834451-3834729 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T214195 NZ_CP110015:c418863-418645 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT214195 WP_000729703.1 NZ_CP082129:3834188-3834448 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT214195 NZ_CP110015:c419263-418889 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References