Detailed information of TA system TA214194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3827991..3828685
• Replicon: chromosome
• Accession: NZ_CP082129
• Organism: Escherichia coli strain NDM_11.16372

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: K5P77_RS18510
• Protein ID: WP_001263489.1
• Coordinates: 3827991..3828389 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: K5P77_RS18515
• Protein ID: WP_000554758.1
• Coordinates: 3828392..3828685 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3823579)	3823579..3823659	-	81	NuclAT_13	-	-
- (3823579)	3823579..3823659	-	81	NuclAT_13	-	-
- (3823579)	3823579..3823659	-	81	NuclAT_13	-	-
- (3823579)	3823579..3823659	-	81	NuclAT_13	-	-
K5P77_RS18485 (3824255)	3824255..3824713	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
K5P77_RS18490 (3824974)	3824974..3826431	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
K5P77_RS18495 (3826488)	3826488..3827009	-	522	Protein_3642	peptide chain release factor H	-
K5P77_RS18500 (3827005)	3827005..3827211	-	207	Protein_3643	RtcB family protein	-
K5P77_RS18505 (3827529)	3827529..3827981	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
K5P77_RS18510 (3827991)	3827991..3828389	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
K5P77_RS18515 (3828392)	3828392..3828685	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
K5P77_RS18520 (3828737)	3828737..3829792	-	1056	WP_001226164.1	DNA polymerase IV	-
K5P77_RS18525 (3829863)	3829863..3830648	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
K5P77_RS18530 (3830620)	3830620..3832332	+	1713	Protein_3649	flagellar biosynthesis protein FlhA	-
K5P77_RS18535 (3832556)	3832556..3833053	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T214194 WP_001263489.1 NZ_CP082129:c3828389-3827991 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T214194 NZ_CP110015:c236998-236657 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT214194 WP_000554758.1 NZ_CP082129:c3828685-3828392 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT214194 NZ_CP110015:c237336-237019 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3