Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1928644..1929146 Replicon chromosome
Accession NZ_CP082129
Organism Escherichia coli strain NDM_11.16372

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag K5P77_RS09185 Protein ID WP_000767829.1
Coordinates 1928892..1929146 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag K5P77_RS09180 Protein ID WP_001259255.1
Coordinates 1928644..1928895 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P77_RS09155 (1923822) 1923822..1924889 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
K5P77_RS09160 (1924889) 1924889..1925959 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
K5P77_RS09165 (1925962) 1925962..1927260 - 1299 WP_087760826.1 histidinol dehydrogenase -
K5P77_RS09170 (1927266) 1927266..1928165 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
K5P77_RS09175 (1928311) 1928311..1928361 - 51 WP_001364200.1 his operon leader peptide -
K5P77_RS09180 (1928644) 1928644..1928895 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
K5P77_RS09185 (1928892) 1928892..1929146 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
K5P77_RS09190 (1929229) 1929229..1930053 + 825 WP_000754762.1 SDR family oxidoreductase -
K5P77_RS09195 (1930099) 1930099..1931028 + 930 WP_087760827.1 LysR substrate-binding domain-containing protein -
K5P77_RS09200 (1931243) 1931243..1931305 + 63 WP_010723108.1 membrane protein YoeI -
K5P77_RS09205 (1931295) 1931295..1932653 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
K5P77_RS09210 (1932832) 1932832..1933890 + 1059 WP_000492324.1 thiosulfate utilization transporter TsuA/YeeE -
K5P77_RS09215 (1933904) 1933904..1934131 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T214181 WP_000767829.1 NZ_CP082129:1928892-1929146 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T214181 NZ_CP110014:2857039-2858112 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT214181 WP_001259255.1 NZ_CP082129:1928644-1928895 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT214181 NZ_CP110014:2858105-2858476 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References