Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3834076..3834617 Replicon chromosome
Accession NZ_CP082127
Organism Escherichia coli strain NDM_12_14482

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag K5P76_RS18545 Protein ID WP_000615983.1
Coordinates 3834339..3834617 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag K5P76_RS18540 Protein ID WP_000729703.1
Coordinates 3834076..3834336 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P76_RS18520 (3829751) 3829751..3830536 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
K5P76_RS18525 (3830508) 3830508..3832220 + 1713 Protein_3649 flagellar biosynthesis protein FlhA -
K5P76_RS18530 (3832444) 3832444..3832941 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
K5P76_RS18535 (3833117) 3833117..3833866 - 750 WP_000056849.1 C40 family peptidase -
K5P76_RS18540 (3834076) 3834076..3834336 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K5P76_RS18545 (3834339) 3834339..3834617 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K5P76_RS18550 (3834773) 3834773..3835513 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K5P76_RS18555 (3835484) 3835484..3836251 - 768 WP_000333380.1 class II glutamine amidotransferase -
K5P76_RS18560 (3836457) 3836457..3837035 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T214164 WP_000615983.1 NZ_CP082127:3834339-3834617 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T214164 NZ_CP110003:63972-64535 [Flavobacterium sp. N2155]
ATGATTCATAGTGTTCGATACAAAGCAGTTCTTGATACCAATGTAATATTTCCTATCGTGATTAGAGATTTATTATTTTG
GTTTGCTCATTATGATTTATATACCCCAAAATGGAGTAAAATCATTTTTGATGAATGGAAATCTGTAATGATTCGAAAGG
GAGTAGAATCTTCACAAGCAGAGAAGCGGATAAACATAGCAAATTTGGCATTTCCCGATGCTTTAGTATTAAATTATGAA
GGTTTAATTGATACTTTAAGTTTACCTGATAAAGATGATAGACATGTATTAGCAGCTGCTATTAAAGTCAATGCCAATAT
TATTGTAACTAATAACTTGAAAGATTTTCCAGATGATTATTTAGACTCTTTTGGATTGAAGGCAGTTTGTGCGGATGATT
TTCTTACGGATATCATTGATTTAAATCCAAAAATAGCCCTTCAAGCTTTTAAAGATTTGGTATTAAATCGCAAAAACCCG
GCAATGGATGAATTTCAAGTATTAGAAAGTTTTAGAAAAAATGGATTAAATCAAACAGCAGATTATTTACATGCTTTATT
GTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT214164 WP_000729703.1 NZ_CP082127:3834076-3834336 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT214164 NZ_CP110003:63527-63982 [Flavobacterium sp. N2155]
ATGGAAGCATTAGATTATATAAAAAGACCCACTAAGAAAGAACAACGAGTGGCTATTGAATCTTATTCAGCACTTTCTTC
AATTTTATCACAATTGAAGAAAGATGATACTGAAATAGAAATCGAAGAAACAAACGACAGAATTAAAATACCTTTTAAAG
CTTTAAAACTTTTAAGTGAAATTTTAAAGTCAATGAGTGAAGGAAATCCAATTTCTATTGTACCTATTGCAACGGAAGTC
ACTACTCAAAAAGCAGCTGAAATATTGGGTTGTTCAAGGCCTCATTTAGTTAAACTTTTAGAACAAGGCGAAATTGATTT
TACAATGGTTGGAAGACATCGTCGTATTTTATTTGAAGATGTAATCAAATATAAAAACGAAATGAAGCAAAAACAGAAAA
AGCATATCATTGATATTATGAACTTTGATGATGAAATAGGTTTATATGATTCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References