Detailed information of TA system TA214163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3827879..3828573
• Replicon: chromosome
• Accession: NZ_CP082127
• Organism: Escherichia coli strain NDM_12_14482

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: K5P76_RS18505
• Protein ID: WP_001263489.1
• Coordinates: 3827879..3828277 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: K5P76_RS18510
• Protein ID: WP_000554758.1
• Coordinates: 3828280..3828573 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3823467)	3823467..3823547	-	81	NuclAT_13	-	-
- (3823467)	3823467..3823547	-	81	NuclAT_13	-	-
- (3823467)	3823467..3823547	-	81	NuclAT_13	-	-
- (3823467)	3823467..3823547	-	81	NuclAT_13	-	-
K5P76_RS18480 (3824143)	3824143..3824601	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
K5P76_RS18485 (3824862)	3824862..3826319	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
K5P76_RS18490 (3826376)	3826376..3826897	-	522	Protein_3642	peptide chain release factor H	-
K5P76_RS18495 (3826893)	3826893..3827099	-	207	Protein_3643	RtcB family protein	-
K5P76_RS18500 (3827417)	3827417..3827869	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
K5P76_RS18505 (3827879)	3827879..3828277	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
K5P76_RS18510 (3828280)	3828280..3828573	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
K5P76_RS18515 (3828625)	3828625..3829680	-	1056	WP_001226164.1	DNA polymerase IV	-
K5P76_RS18520 (3829751)	3829751..3830536	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
K5P76_RS18525 (3830508)	3830508..3832220	+	1713	Protein_3649	flagellar biosynthesis protein FlhA	-
K5P76_RS18530 (3832444)	3832444..3832941	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T214163 WP_001263489.1 NZ_CP082127:c3828277-3827879 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T214163 NZ_CP110002:c235428-235129 [Flavobacterium sp. N2155]
ATGGGGTATAAAATCAGTCATGAAGCGAATCGGGATATTGAGAACATTTGGCTTTATACATATGAAAACTGGTCGCTCGA
ACAAGCGGATAGATACATCAACCTGATAATTGACGAAATAGAATACTTAGCGGAAAATCCAAAATCAGGAAAAGATTATG
GGCATCTAAGAAAAGGCTATTTTCGTTCTCAAATAAAATCACATTTCATTTTCTATAAAATCAGTCTAAAAAGAGAAGAA
ATTGAAATCATCAGAGTTTTGCATCAAAGAATGGATATTGAATCCCGACTGACTGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT214163 WP_000554758.1 NZ_CP082127:c3828573-3828280 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT214163 NZ_CP110002:c235676-235428 [Flavobacterium sp. N2155]
ATGGCTAAAAACACATCTATTTTGCTCGGGGATTATTTTGACAATTTTATCAGCCGACAAATTAAAACAGGAAAATATTC
TTCCGCAAGTGAAGTTGTTAGAGCGGCTTTGAGAATGTTCGAGTACGAAGAGTCAAAAAGAACCGAACTGATAAAGGAAC
TGAAAAAAGGAGAAAAATCAGGTTTTGTAAAAAACTTTGACAGAAAAGAACTTTTAAATAGCCTTCATCAAAAGCATTTG
AGTAAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3