Detailed information of TA system TA021416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3397941..3398638
• Replicon: chromosome
• Accession: NC_007777
• Organism: Frankia sp. CcI3

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: FRANCCI3_RS14480
• Protein ID: WP_070982730.1
• Coordinates: 3397941..3398312 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FRANCCI3_RS14485
• Protein ID: WP_011437269.1
• Coordinates: 3398309..3398638 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRANCCI3_RS14450	3393330..3393914	-	585	WP_011437263.1	superoxide dismutase family protein	-
FRANCCI3_RS14455	3394252..3394641	-	390	WP_011437264.1	type II toxin-antitoxin system VapC family toxin	-
FRANCCI3_RS14460	3394638..3394925	-	288	WP_023841118.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
FRANCCI3_RS14465	3394873..3395712	-	840	WP_011437265.1	5,6-dimethylbenzimidazole synthase	-
FRANCCI3_RS14470	3396011..3396392	-	382	Protein_2948	helix-turn-helix transcriptional regulator	-
FRANCCI3_RS14475	3396520..3397491	-	972	WP_011437267.1	Rpn family recombination-promoting nuclease/putative transposase	-
FRANCCI3_RS14480	3397941..3398312	+	372	WP_070982730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FRANCCI3_RS14485	3398309..3398638	+	330	WP_011437269.1	helix-turn-helix domain-containing protein	Antitoxin
FRANCCI3_RS14490	3398815..3399258	+	444	Protein_2952	transposase	-
FRANCCI3_RS14495	3399255..3400943	-	1689	WP_011437271.1	IS66-like element ISFsp11 family transposase	-
FRANCCI3_RS14500	3401428..3401652	-	225	WP_023841125.1	hypothetical protein	-
FRANCCI3_RS14505	3401649..3402176	-	528	WP_011437272.1	NUDIX hydrolase	-
FRANCCI3_RS14510	3402269..3402577	+	309	WP_023841126.1	hypothetical protein	-
FRANCCI3_RS14515	3402796..3403545	-	750	WP_011437274.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3399255..3400943	1688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13831.56 Da
• Isoelectric Point: 4.4605

>T21416 WP_070982730.1 NC_007777:3397941-3398312 [Frankia casuarinae]
VDEWEVRVTNEFLAWVNELDPRSRALVVDAIDRLADAGPSLGRPLVDRLVGSEIHNLKELRPASAGTSEIRVLFVFDPWR
CAILLVGGDKSGDWSGWYRTAIPRAETLYAEYMKEREAEEGSS

Nucleotide

• Length: 372 bp

>T21416 NC_007777:3397941-3398312 [Frankia casuarinae]
GTGGACGAATGGGAAGTCCGCGTGACGAACGAGTTCCTGGCCTGGGTCAACGAGCTCGATCCGAGGTCCAGGGCGCTCGT
CGTCGACGCGATCGATCGGCTGGCCGATGCCGGGCCAAGCCTGGGCCGACCACTGGTCGACCGCCTGGTCGGCTCGGAGA
TCCACAATCTCAAGGAGCTCAGGCCAGCTTCCGCGGGCACCTCGGAGATCCGCGTCCTGTTCGTCTTCGATCCCTGGCGC
TGCGCCATCCTGCTGGTCGGTGGGGACAAGTCGGGCGACTGGTCCGGCTGGTACCGCACGGCGATCCCACGCGCGGAGAC
GCTGTACGCCGAGTACATGAAGGAACGGGAAGCCGAGGAGGGCAGCTCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12240.77 Da
• Isoelectric Point: 6.4725

>AT21416 WP_011437269.1 NC_007777:3398309-3398638 [Frankia casuarinae]
MTRFPRWNDVRADLIADAEETLTEARRRNQAYIDGHRLAERRRLLGLTQTEVAEHMGVSKSRVSQIERGDVSTVDVIARY
VQALGGQIQITAVFGDDLYILRGTDTPAA

Nucleotide

• Length: 330 bp

>AT21416 NC_007777:3398309-3398638 [Frankia casuarinae]
ATGACCAGATTCCCCCGGTGGAACGACGTCCGCGCCGATCTGATCGCCGACGCCGAGGAGACCCTGACCGAGGCCCGCAG
GCGCAACCAGGCCTACATCGACGGCCACCGCCTCGCTGAACGCCGCAGGCTCCTCGGCCTGACCCAGACCGAGGTCGCCG
AGCACATGGGTGTCAGCAAGAGCCGGGTCTCCCAGATCGAACGCGGCGACGTCTCCACCGTCGACGTCATCGCCCGCTAC
GTCCAGGCCCTCGGCGGCCAGATCCAGATCACCGCCGTCTTCGGCGACGATCTCTACATCCTGCGCGGCACCGACACGCC
CGCTGCGTGA