Detailed information of TA system TA214146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 754857..755550
• Replicon: chromosome
• Accession: NZ_CP082127
• Organism: Escherichia coli strain NDM_12_14482

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: K5P76_RS03655
• Protein ID: WP_000415584.1
• Coordinates: 754857..755153 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: K5P76_RS03660
• Protein ID: WP_000650107.1
• Coordinates: 755155..755550 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P76_RS03620 (749945)	749945..750259	-	315	WP_000958598.1	putative quinol monooxygenase	-
K5P76_RS03625 (750290)	750290..750871	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
K5P76_RS03630 (751190)	751190..751522	+	333	WP_000917685.1	DUF2645 family protein	-
K5P76_RS03635 (751568)	751568..752917	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
K5P76_RS03640 (752914)	752914..753573	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
K5P76_RS03645 (753725)	753725..754117	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
K5P76_RS03650 (754170)	754170..754652	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
K5P76_RS03655 (754857)	754857..755153	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
K5P76_RS03660 (755155)	755155..755550	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
K5P76_RS03665 (755683)	755683..757290	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
K5P76_RS03670 (757428)	757428..759686	+	2259	WP_032329482.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T214146 WP_000415584.1 NZ_CP082127:754857-755153 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T214146 NZ_CP109983:c4824777-4824400 [Klebsiella pneumoniae]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT214146 WP_000650107.1 NZ_CP082127:755155-755550 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT214146 NZ_CP109983:c4825235-4824867 [Klebsiella pneumoniae]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCATGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65