Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 649958..650757 Replicon chromosome
Accession NZ_CP082127
Organism Escherichia coli strain NDM_12_14482

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag K5P76_RS03135 Protein ID WP_000347275.1
Coordinates 649958..650422 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K5P76_RS03140 Protein ID WP_001307405.1
Coordinates 650422..650757 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P76_RS03105 (644959) 644959..645393 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K5P76_RS03110 (645411) 645411..646289 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K5P76_RS03115 (646279) 646279..647058 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
K5P76_RS03120 (647069) 647069..647542 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
K5P76_RS03125 (647565) 647565..648845 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
K5P76_RS03130 (649094) 649094..649903 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K5P76_RS03135 (649958) 649958..650422 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K5P76_RS03140 (650422) 650422..650757 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K5P76_RS03145 (650906) 650906..652477 - 1572 WP_001273752.1 galactarate dehydratase -
K5P76_RS03150 (652852) 652852..654186 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
K5P76_RS03155 (654202) 654202..654972 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T214144 WP_000347275.1 NZ_CP082127:c650422-649958 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T214144 NZ_CP109983:4151910-4152128 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT214144 WP_001307405.1 NZ_CP082127:c650757-650422 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT214144 NZ_CP109983:4151509-4151883 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References