Detailed information of TA system TA214139

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43527..43791
• Replicon: plasmid pIncI
• Accession: NZ_CP082123
• Organism: Escherichia coli strain PC-NDM34

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: K5P75_RS24930
• Protein ID: WP_001387489.1
• Coordinates: 43527..43679 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	43731..43791 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P75_RS24895 (38619)	38619..39152	-	534	WP_001221666.1	lipocalin family protein	-
K5P75_RS24900 (39246)	39246..40391	-	1146	WP_039023136.1	extended-spectrum class C beta-lactamase CMY-42	-
K5P75_RS25575 (41596)	41596..41694	+	99	Protein_48	ethanolamine utilization protein EutE	-
K5P75_RS24910 (41766)	41766..41975	-	210	WP_134892286.1	HEAT repeat domain-containing protein	-
K5P75_RS24915 (42367)	42367..42543	+	177	WP_001054897.1	hypothetical protein	-
K5P75_RS24920 (42608)	42608..42904	-	297	WP_011264046.1	DinQ-like type I toxin DqlB	-
K5P75_RS24925 (43204)	43204..43455	+	252	WP_001291964.1	hypothetical protein	-
K5P75_RS24930 (43527)	43527..43679	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (43731)	43731..43791	+	61	NuclAT_0	-	Antitoxin
- (43731)	43731..43791	+	61	NuclAT_0	-	Antitoxin
- (43731)	43731..43791	+	61	NuclAT_0	-	Antitoxin
- (43731)	43731..43791	+	61	NuclAT_0	-	Antitoxin
K5P75_RS24935 (44000)	44000..44374	+	375	WP_223349261.1	hypothetical protein	-
K5P75_RS24940 (44527)	44527..45684	+	1158	Protein_55	IS1380-like element ISEcp1 family transposase	-
K5P75_RS24945 (45740)	45740..46437	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
K5P75_RS24950 (46448)	46448..46756	-	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
K5P75_RS24955 (47198)	47198..47485	-	288	WP_000074855.1	conjugal transfer protein TraA	-
K5P75_RS25580 (47523)	47523..47681	-	159	Protein_59	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..48592	48592
	-	inside	IScluster/Tn	blaCMY-42	-	39246..46015	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T214139 WP_001387489.1 NZ_CP082123:c43679-43527 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T214139 NZ_CP109983:713216-713557 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATTACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATACGGACTGGTTCGTATCGACAGGAATGGATTT
GTCTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCGCGGCAGGCAACTGGCTTATTGCGACAAAG
CCGTAACAACGCAATACGTTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT214139 NZ_CP082123:43731-43791 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin