Detailed information of TA system TA214134

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44841..45080
• Replicon: plasmid pIncFIA
• Accession: NZ_CP082121
• Organism: Escherichia coli strain PC-NDM34

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: K5P75_RS23745
• Protein ID: WP_023144756.1
• Coordinates: 44841..44975 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	45020..45080 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P75_RS23715 (40542)	40542..41399	-	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
K5P75_RS23720 (41392)	41392..41466	-	75	WP_014966221.1	RepA leader peptide Tap	-
K5P75_RS23725 (41466)	41466..41597	-	132	Protein_55	protein CopA/IncA	-
K5P75_RS23730 (41828)	41828..43369	+	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
K5P75_RS23735 (43384)	43384..44130	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
K5P75_RS23740 (44290)	44290..44544	-	255	WP_000083850.1	replication regulatory protein RepA	-
K5P75_RS23745 (44841)	44841..44975	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (45020)	45020..45080	+	61	NuclAT_1	-	Antitoxin
- (45020)	45020..45080	+	61	NuclAT_1	-	Antitoxin
- (45020)	45020..45080	+	61	NuclAT_1	-	Antitoxin
- (45020)	45020..45080	+	61	NuclAT_1	-	Antitoxin
K5P75_RS23750 (45047)	45047..45333	-	287	Protein_60	DUF2726 domain-containing protein	-
K5P75_RS23755 (45846)	45846..46058	-	213	WP_013023861.1	hypothetical protein	-
K5P75_RS23760 (46189)	46189..46749	-	561	WP_000139341.1	fertility inhibition protein FinO	-
K5P75_RS23765 (46804)	46804..47550	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / aac(6')-Ib-cr / dfrA17 / aadA5 / qacE / mph(A) / blaTEM-1B / aac(3)-IId / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..109135	109135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T214134 WP_023144756.1 NZ_CP082121:c44975-44841 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T214134 NZ_CP109974:c111703-111260 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT214134 NZ_CP082121:45020-45080 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin