Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3804895..3805436 Replicon chromosome
Accession NZ_CP082120
Organism Escherichia coli strain PC-NDM34

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag K5P75_RS18495 Protein ID WP_000615976.1
Coordinates 3805158..3805436 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag K5P75_RS18490 Protein ID WP_000729704.1
Coordinates 3804895..3805155 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P75_RS18465 (3800678) 3800678..3801463 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
K5P75_RS18470 (3801435) 3801435..3803147 + 1713 Protein_3624 flagellar biosynthesis protein FlhA -
K5P75_RS18475 (3803252) 3803252..3803530 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
K5P75_RS18480 (3803523) 3803523..3803879 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
K5P75_RS18485 (3803936) 3803936..3804709 - 774 WP_000543895.1 C40 family peptidase -
K5P75_RS18490 (3804895) 3804895..3805155 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K5P75_RS18495 (3805158) 3805158..3805436 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K5P75_RS18500 (3805592) 3805592..3806332 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K5P75_RS18505 (3806303) 3806303..3807070 - 768 WP_000333380.1 class II glutamine amidotransferase -
K5P75_RS18510 (3807276) 3807276..3807854 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T214130 WP_000615976.1 NZ_CP082120:3805158-3805436 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T214130 NZ_CP109973:c5362228-5361845 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT214130 WP_000729704.1 NZ_CP082120:3804895-3805155 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT214130 NZ_CP109973:c5362470-5362228 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References