Detailed information of TA system TA214117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1857390..1857892
• Replicon: chromosome
• Accession: NZ_CP082120
• Organism: Escherichia coli strain PC-NDM34

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A8E0FJZ4
• Locus tag: K5P75_RS08865
• Protein ID: WP_000767833.1
• Coordinates: 1857638..1857892 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: K5P75_RS08860
• Protein ID: WP_001259255.1
• Coordinates: 1857390..1857641 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P75_RS08835 (1852569)	1852569..1853636	-	1068	WP_032202492.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
K5P75_RS08840 (1853636)	1853636..1854706	-	1071	WP_000108970.1	histidinol-phosphate transaminase	-
K5P75_RS08845 (1854703)	1854703..1856007	-	1305	WP_058905757.1	histidinol dehydrogenase	-
K5P75_RS08850 (1856013)	1856013..1856912	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
K5P75_RS08855 (1857058)	1857058..1857108	-	51	WP_001364200.1	his operon leader peptide	-
K5P75_RS08860 (1857390)	1857390..1857641	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
K5P75_RS08865 (1857638)	1857638..1857892	+	255	WP_000767833.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
K5P75_RS08870 (1857975)	1857975..1858799	+	825	WP_032202495.1	SDR family oxidoreductase	-
K5P75_RS08875 (1858845)	1858845..1859774	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
K5P75_RS08880 (1859989)	1859989..1860051	+	63	WP_010723108.1	membrane protein YoeI	-
K5P75_RS08885 (1860041)	1860041..1861399	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
K5P75_RS08890 (1861578)	1861578..1862636	+	1059	WP_000492312.1	thiosulfate utilization transporter TsuA/YeeE	-
K5P75_RS08895 (1862650)	1862650..1862877	+	228	WP_000985278.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10173.54 Da
• Isoelectric Point: 8.0353

>T214117 WP_000767833.1 NZ_CP082120:1857638-1857892 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T214117 NZ_CP109968:580552-580911 [Agrobacterium salinitolerans]
ATGGTCCGCAGTCAGGTTCCAAAACGTGGCGACGTTTATCTGGTCGACCTCAATCCTGTGATCGGCAGCGAAATCAGGGA
CGAACATCGTTGTATCGTCATCACGCCCAGAGAAATCAACGCGGTTGGACTGTGCCTTGTCGTTCCCGTGAACACGGGCG
GACTATTCACGCGCAGGGCGGGGCTTGCCGTGAACATATCGGGCCATAAGACAACCGGTGTCGCACTATGCAACCAGGTG
AGAAGCATGGACATTGTTGCCCGTGCCTCACAGAAGAAAGCGAAATATATCGAAACCCTTGACGATGCTACGATCGACAA
AATCGTGGGTCGTGTCATCAGCATGATCGACCCCGCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT214117 WP_001259255.1 NZ_CP082120:1857390-1857641 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT214117 NZ_CP109968:580286-580552 [Agrobacterium salinitolerans]
ATGACCGTAACGACGAGAATAAGACGTCAGGGCGGTGCGGCGGTGATGACCATCCCGCCCGCTCTTTTGAAAATGCTGGG
ACTGGAAATCGGTGAGCAATTGACGCTGGAGGTCGATAACGGCGCGCTGGTTGCAAGCCCTGTTCGGCAGGAAAAGAAAC
GCTTCACGCTTGCCGAATTGCTGGAGGGGGCGGATGAAGTCGCCGCTCTCAATGCCAGCGCGCGGGAATGGGACGAAGCG
CCGCCAGTCGGCAAAGAAGCTCTTTGA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0